Fas, FasL, and cleaved caspases 8 and 3 in glioblastomas: A tissue microarray-based study

Fas, FasL, and cleaved caspases 8 and 3 in glioblastomas: A tissue microarray-based study

Author Saggioro, Fabiano Pinto Google Scholar
Neder, Luciano Google Scholar
Stávale, João Norberto Autor UNIFESP Google Scholar
Paixão-Becker, Aline Nazareth de Paiva Google Scholar
Malheiros, Suzana Maria Fleury Autor UNIFESP Google Scholar
Soares, Fernando Augusto Google Scholar
Pittella, José Eymard Homem Google Scholar
Matias, Caio César Marconato Simões Google Scholar
Colli, Benedicto Oscar Google Scholar
Carlotti Junior, Carlos Gilberto Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Hosp Canc AC Camargo
Abstract This investigation analyzed the immunoexpression of FasL, Fas, cleaved caspase-8, and cleaved caspase-3 in glioblastomas. Formalin-fixed and paraffin-embedded glioblastoma tissues and control brain tissues from 97 patients were analyzed by tissue microarrays and immunohistochemistry. Patients with glioblastomas that were negative or weakly stained (<50% of cells positive) for cleaved caspase-8 had worse cancer-specific overall survival (median=8.5 months) than did patients with tumors that highly expressed cleaved caspase-8 (median=11.7 months; P=0.0325), independent of clinical variables. There was no association of other markers with survival, treatment, sex, age, tumor size, and primary site. Among the tumors, there were reasonable to good positive correlations between the expression of FasL and Fas (r=0.47) and between Fas and cleaved caspase-8 (r=0.41), and there were poor positive correlations between Fas and cleaved caspase-3 (r=0.26), FasL and cleaved caspase-8 (r=0.22), and cleaved caspase-8 and -3 (r=0.31). Our results suggest that Fas-Fas-ligand signal transduction could be inhibited, especially at the stage of caspase-8 activation, thereby establishing a major mechanism for evasion of apoptosis by these tumors. the absence or low expression of cleaved caspase-8 in the tumors was a negative prognostic indicator for patient survival. (C) 2014 Elsevier GmbH. All rights reserved.
Keywords Glioblastoma
Cleaved caspase 8
Apoptosis
Survival
Caspase 3
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/09932-4
Date 2014-01-01
Published in Pathology Research and Practice. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 210, n. 5, p. 267-273, 2014.
ISSN 0344-0338 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 267-273
Origin http://dx.doi.org/10.1016/j.prp.2013.12.012
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000335206600001
URI http://repositorio.unifesp.br/handle/11600/37117

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