Grape juice concentrate protects reproductive parameters of male rats against cadmium-induced damage: a chronic assay

Grape juice concentrate protects reproductive parameters of male rats against cadmium-induced damage: a chronic assay

Author Pires, Vanessa Cardoso Autor UNIFESP Google Scholar
Boiago Golluecke, Andrea Pittelli Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Lungato, Lisandro Autor UNIFESP Google Scholar
D'Almeida, Vania Autor UNIFESP Google Scholar
Aguiar, Odair Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Catolica Santos
Abstract The aim of the present study was to investigate the effects of long-term grape juice concentrate (GJC) consumption, in two dosages, on the reproductive parameters of cadmium-exposed male rats. the effects of the concentrate on body mass gain, plasma testosterone levels, reproductive organ weights, daily sperm production, sperm morphology, testis histopathological and histomorphometrical parameters, and testicular antioxidant markers were investigated. Wistar rats (n 54) were distributed into six groups: CdCl2; cadmium and grape juice I (1.18 g/kg per d); cadmium and grape juice II (2.36 g/kg per d); grape juice I (1.18 g/kg per d); grape juice II (2.36 g/kg per d); control. A single dose of CdCl2 (1.2 mg/kg body weight (BW)) was injected intraperitoneally and the grape juice was administered orally for 56 d. the results indicated that cadmium changed all reproductive and antioxidant parameters. At dosage I (1.18 g/kg BW), GJC consumption did not show the effects against cadmium-induced damages. in contrast, at dosage II (2.36 g/kg BW), the GJC improved the gonadosomatic index (P=0.003), serum testosterone levels (P=0.001), the relative weight of epididymis (P=0.013) and ventral prostate (P=0.052), the percentage of normal sperm (P=0.001), and histopathological and histomorphometrical parameters. in addition, at this dosage, normalisation of the enzymatic activity of superoxide dismutase (P=0.001) and of testicular levels of glutathione (P=0.03) were observed. the parameters of the non-exposed rats did not depict significant alterations. in conclusion, the product was able to act as a protector of reproductive function against cadmium-induced damage. Such a property was expressed in a dose-dependent manner as the more effective dose was dosage II. the GJC acted possibly by antioxidant mechanisms.
Keywords Functional foods
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2011/22811-5
FAPESP: 2011/03873-0
Date 2013-12-14
Published in British Journal of Nutrition. Cambridge: Cambridge Univ Press, v. 110, n. 11, p. 2020-2029, 2013.
ISSN 0007-1145 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 2020-2029
Access rights Closed access
Type Article
Web of Science ID WOS:000330345900011

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