Tooth Tissue Engineering: the Influence of Hydrophilic Surface on Nanocrystalline Diamond Films for Human Dental Stem Cells

Tooth Tissue Engineering: the Influence of Hydrophilic Surface on Nanocrystalline Diamond Films for Human Dental Stem Cells

Author Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Ladislau Carvalho Salmazi, Karina Inacio Google Scholar
Salvadori, Maria Cecilia Google Scholar
Teixeira, Fernanda de Sa Google Scholar
Pasquarelli, Alberto Google Scholar
Vacanti, Joseph Phillip Google Scholar
Yelick, Pamela Crotty Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
INCT Biofabris
Universidade de São Paulo (USP)
Univ Ulm
Massachusetts Gen Hosp
Harvard Univ
Tufts Univ
Abstract New techniques for tissue engineering (TE) are rapidly emerging. the basic concept of autologous TE is to isolate cells from small biopsy specimens, and to expand these cells in culture for subsequent seeding onto biodegradable scaffolds. Nanocrystalline diamond films have attracted the attention of researchers from a variety of different areas in recent years, due to their unique and exceptional properties. in this approach, human dental stem cells (hDSCs) were characterized by flow cytometry and grown on diamond films with hydrogen (H)-terminated and oxygen (O)-terminated surfaces for 28 days, and then removed by lysis and washing with distilled water. Energy dispersive spectroscopy analysis was performed, showing that the regions with O-terminated surfaces contained much higher levels of deposited calcium, oxygen, and phosphorus. These results suggest that the extracellular matrix was considerably more developed in the O-terminated regions, as compared with the H-terminated regions. in addition, optical microscopy of hDSCs cultured on the diamond substrate with H- and O-terminated surfaces, before washing with distilled water, showed preferential directions of the cells arrangement, where orthogonal lines suggest that the cells appeared to be following the O-terminated regions or hydrophilic surface. These findings suggest that O-terminated diamond surfaces prepared on biodegradable scaffolds can be useful for mineralized dental tissue formation.
Language English
Sponsor UNIFESP
Plastic Surgery Department
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Instituto Nacional de Ciencia e Tecnologia (INCT)-Biofabrication
Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED
NIH/NIDCR/NIBIB
Grant number Instituto Nacional de Ciencia e Tecnologia (INCT)-Biofabrication: CNPq 5736661/2008-1
Instituto Nacional de Ciencia e Tecnologia (INCT)-Biofabrication: FAPESP 08/57860-3
Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED: 208RT0340
NIH/NIDCR/NIBIB: DE016132
NIH/NIDCR/NIBIB: TW007665
CNPq: 310048/2011-7 e 310049/2011-3
Date 2013-12-01
Published in Tissue Engineering Part A. New Rochelle: Mary Ann Liebert, Inc, v. 19, n. 23-24, p. 2537-2543, 2013.
ISSN 1937-3341 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert, Inc
Extent 2537-2543
Origin http://dx.doi.org/10.1089/ten.tea.2012.0628
Access rights Closed access
Type Article
Web of Science ID WOS:000328333700001
URI http://repositorio.unifesp.br/handle/11600/37056

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