Purification and Characterization of an Ethanol-Tolerant beta-Glucosidase from Sporidiobolus pararoseus and Its Potential for Hydrolysis of Wine Aroma Precursors

Purification and Characterization of an Ethanol-Tolerant beta-Glucosidase from Sporidiobolus pararoseus and Its Potential for Hydrolysis of Wine Aroma Precursors

Author Baffi, Milla Alves Google Scholar
Martin, Natalia Google Scholar
Tobal, Thaise Maria Google Scholar
Ferrarezi, Ana Lucia Google Scholar
Ghilardi Lago, Joao Henrique Autor UNIFESP Google Scholar
Boscolo, Mauricio Google Scholar
Gomes, Eleni Google Scholar
Da-Silva, Roberto Google Scholar
Institution Universidade Federal de Uberlândia (UFU)
São Paulo State Univ UNESP
Universidade Federal de São Paulo (UNIFESP)
Abstract An extracellular ethanol-tolerant beta-glucosidase from Sporidiobolus pararoseus was purified to homogeneity and characterized, and its potential use for the enhancement of wine aroma was investigated. the crude enzymatic extract was purified in four steps (concentration, dialysis, ultrafiltration, and chromatography) with a yield of around 40 % for total activity. the purified enzyme (designated Sp-beta gl-P) showed a specific activity of approximately 20.0 U/mg, an estimated molecular mass of 63 kDa after sodium dodecyl sulfate polyacrylamide gel electrophoresis, and isoelectric point of 5.0 by isoelectric focusing. Sp-beta gl-P has optimal activity at pH 4.0 and at 55 A degrees C. It was stable in a broad pH range at low temperatures and it was tolerant to ethanol and glucose, indicating suitable properties for winemaking. the hydrolysis of glycosidic terpenes was analyzed by adding Sp-beta gl-P directly to the wines. the released terpene compounds were evaluated by gas chromatography/mass spectrometry. the enzymatic treatment significantly increased the amount of free terpenes, suggesting that this enzyme could potentially be applicable in wine aroma improvement.
Keywords Yeasts
beta-Glucosidase
Purification
Wine
Terpenes
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-12-01
Published in Applied Biochemistry and Biotechnology. Totowa: Humana Press Inc, v. 171, n. 7, p. 1681-1691, 2013.
ISSN 0273-2289 (Sherpa/Romeo, impact factor)
Publisher Humana Press Inc
Extent 1681-1691
Origin http://dx.doi.org/10.1007/s12010-013-0471-0
Access rights Closed access
Type Article
Web of Science ID WOS:000327479800012
URI http://repositorio.unifesp.br/handle/11600/37033

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