Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii

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dc.contributor.author Oliveira, Miriam Pires de Castro [UNIFESP]
dc.contributor.author Ramos, Thiago Cesar Prata [UNIFESP]
dc.contributor.author Pinheiro, Adriana Maria Viana Nunes [UNIFESP]
dc.contributor.author Bertini, Silvio [UNIFESP]
dc.contributor.author Takahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.author Straus, Anita Hilda [UNIFESP]
dc.contributor.author Freymüller-Haapalainen, Edna [UNIFESP]
dc.date.accessioned 2016-01-24T14:34:44Z
dc.date.available 2016-01-24T14:34:44Z
dc.date.issued 2013-12-01
dc.identifier http://dx.doi.org/10.1016/j.actatropica.2013.08.001
dc.identifier.citation Acta Tropica. Amsterdam: Elsevier B.V., v. 128, n. 3, p. 548-556, 2013.
dc.identifier.issn 0001-706X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36993
dc.description.abstract Trypanosoma (Schizotrypanum) dionisii is a non-pathogenic bat trypanosome closely related to Trypanosoma cruzi, the etiological agent of Chaga's disease. Both kinetoplastids present similar morphological stages and are able to infect mammalian cells in culture. in the present study we examined 3D ultrastructure aspects of the two species by serial sectioning epimastigote and trypomastigote forms, and identified common carbohydrate epitopes expressed in T. dionisii, T. cruzi and Leishmania major. A major difference in 3D morphology was that T. dionisii epimastigote forms present larger multivesicular structures, restricted to the parasite posterior region. These structures could be related to T. cruzi reservosomes and are also rich in cruzipain, the major cysteine-proteinase of T. cruzi. We analyzed the reactivity of two monoclonal antibodies: MEST-1 directed to galactofuranose residues of glycolipids purified from Paracoccidioides brasiliensis, and BST-1 directed to glycolipids purified from T. cruzi epimastigotes. Both antibodies were reactive with T. dionisii epimastigotes by indirect immunofluorescense, but we noted differences in the location and intensity of the epitopes, when compared to T. cruzi. in summary, despite similar features in cellular structure and life cycle of T. dionisii and T. cruzi, we observed a unique morphological characteristic in T. dionisii that deserves to be explored. (C) 2013 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 548-556
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Acta Tropica
dc.rights Acesso aberto
dc.subject Ttypanosoma dionisii en
dc.subject Tridimensional ultrastructure en
dc.subject 3D reconstruction en
dc.subject Epimastigote en
dc.subject Glycolipid en
dc.title Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, BR-04023900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Ctr Microscopia Eletron, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biol Estrutural & Func, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Ctr Microscopia Eletron, BR-04023900 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 06/07005-4
dc.identifier.file WOS000327824800015.pdf
dc.identifier.doi 10.1016/j.actatropica.2013.08.001
dc.description.source Web of Science
dc.identifier.wos WOS:000327824800015



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