Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii

Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii

Author Oliveira, Miriam Pires de Castro Autor UNIFESP Google Scholar
Ramos, Thiago Cesar Prata Autor UNIFESP Google Scholar
Pinheiro, Adriana Maria Viana Nunes Autor UNIFESP Google Scholar
Bertini, Silvio Autor UNIFESP Google Scholar
Takahashi, Helio Kiyoshi Autor UNIFESP Google Scholar
Straus, Anita Hilda Autor UNIFESP Google Scholar
Freymüller-Haapalainen, Edna Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Trypanosoma (Schizotrypanum) dionisii is a non-pathogenic bat trypanosome closely related to Trypanosoma cruzi, the etiological agent of Chaga's disease. Both kinetoplastids present similar morphological stages and are able to infect mammalian cells in culture. in the present study we examined 3D ultrastructure aspects of the two species by serial sectioning epimastigote and trypomastigote forms, and identified common carbohydrate epitopes expressed in T. dionisii, T. cruzi and Leishmania major. A major difference in 3D morphology was that T. dionisii epimastigote forms present larger multivesicular structures, restricted to the parasite posterior region. These structures could be related to T. cruzi reservosomes and are also rich in cruzipain, the major cysteine-proteinase of T. cruzi. We analyzed the reactivity of two monoclonal antibodies: MEST-1 directed to galactofuranose residues of glycolipids purified from Paracoccidioides brasiliensis, and BST-1 directed to glycolipids purified from T. cruzi epimastigotes. Both antibodies were reactive with T. dionisii epimastigotes by indirect immunofluorescense, but we noted differences in the location and intensity of the epitopes, when compared to T. cruzi. in summary, despite similar features in cellular structure and life cycle of T. dionisii and T. cruzi, we observed a unique morphological characteristic in T. dionisii that deserves to be explored. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Ttypanosoma dionisii
Tridimensional ultrastructure
3D reconstruction
Epimastigote
Glycolipid
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 06/07005-4
Date 2013-12-01
Published in Acta Tropica. Amsterdam: Elsevier B.V., v. 128, n. 3, p. 548-556, 2013.
ISSN 0001-706X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 548-556
Origin http://dx.doi.org/10.1016/j.actatropica.2013.08.001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000327824800015
URI http://repositorio.unifesp.br/handle/11600/36993

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