Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy

Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy

Author Cabral, Paula Brito e Google Scholar
Cunha Junior, Jose Evandro Google Scholar
Macedo, Alexandre Casimiro de Google Scholar
Alves, Alexandre Rodrigues Google Scholar
Goncalves, Thially Braga Google Scholar
Cabral, Tereza Cristina Brito e Google Scholar
Gondim, Ana Paula Soares Google Scholar
Moraes-Pinto, Maria Isabel de Autor UNIFESP Google Scholar
Oseki, Karen Tubono Autor UNIFESP Google Scholar
Camara, Lilia Maria Carneiro Google Scholar
Rabenhorst, Silvia Helena Barem Google Scholar
Nagao-Dias, Aparecida Tiemi Autor UNIFESP Google Scholar
Institution Univ Fed Ceara
Universidade Federal de São Paulo (UNIFESP)
Abstract Objectives: Leprosy household contacts represent a group at high risk of developing the disease. the aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.Methods: Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n = 135), their index cases (n = 30), and in persons living in a low endemic city (n = 17).Results: Salivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p < 0.01, p < 0.005, and p < 0.0001, respectively). This was not observed for serum anti-PGL1 IgM (p > 0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p < 0.0001). for IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6%) and in three out of the 50 PB household leprosy contacts (6.0%).Conclusion: We strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts. (C) 2013 International Society for Infectious Diseases. Published by Elsevier B.V. All rights reserved.
Keywords Mycobacterium leprae DNA
Serum and salivary anti-PGL1
Leprosy household contacts
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 472.471/2007-4
Date 2013-11-01
Published in International Journal of Infectious Diseases. Oxford: Elsevier B.V., v. 17, n. 11, p. E1005-E1010, 2013.
ISSN 1201-9712 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent E1005-E1010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000325986700014

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