Gene expression modulation by paraquat-induced oxidative stress conditions in Paracoccidioides brasiliensis

Gene expression modulation by paraquat-induced oxidative stress conditions in Paracoccidioides brasiliensis

Author Oliveira, Marcus Vinicius de Google Scholar
Oliveira, Ana Claudia de Freitas Google Scholar
Shida, Claudio Saburo Autor UNIFESP Google Scholar
Oliveira, Regina Costa de Google Scholar
Nunes, Luiz R. Google Scholar
Institution Univ Mogi das Cruzes
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
Abstract Paracoccidioides brasiliensis is a thermodimorphic fungus associated with paracoccidioidomycosis (PCM), the most common systemic mycosis in Latin America. the infection is initiated by inhalation of environmentally dispersed conidia produced by the saprophytic phase of the fungus. in the lungs, P. brasiliensis assumes the parasitic yeast form and must cope with the adverse conditions imposed by cells of the host immune system, which includes a harsh environment, highly concentrated in reactive oxygen species (ROS). in this work, we used the ROS-generating agent paraquat to experimentally simulate oxidative stress conditions in order to evaluate the stress-induced modulation of gene expression in cultured P. brasiliensis yeast cells, using a microarray hybridization approach. the large-scale evaluation inherent to microarray-based analyses identified 2070 genes differentially transcribed in response to paraquat exposure, allowing an integrated visualization of the major metabolic changes that constitute the systemic defense mechanism used by the fungus to overcome the deleterious effects of ROS. These include overexpression of detoxifying agents, as well as of molecular scavengers and genes involved in maintenance of the intracellular redox potential. Particularly noteworthy was to verify that the oxidative stress resistance mechanism of P. brasiliensis also involves coordinated overexpression of a series of genes responsible for chitin-biosynthesis, suggesting that this pathway may constitute a specific regulon. Further analyses aiming at confirming and understanding the mechanisms that control such regulon may provide interesting new targets for chemotherapeutic approaches against P. brasiliensis and other pathogenic fungi. (C) 2013 Elsevier Inc. All rights reserved.
Keywords Paracoccidioides brasiliensis
Oxidative stress
Gene expression modulation
Antioxidant defense
Chitin synthesis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-11-01
Published in Fungal Genetics and Biology. San Diego: Academic Press Inc Elsevier Science, v. 60, p. 101-109, 2013.
ISSN 1087-1845 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 101-109
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000327290200011

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