Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response

Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response

Author Larocca, Rafael Assumpcao Google Scholar
Moraes-Vieira, Pedro Manoel Google Scholar
Bassi, Enio Jose Google Scholar
Semedo, Patricia Autor UNIFESP Google Scholar
Almeida, Danilo Candido de Autor UNIFESP Google Scholar
Silva, Marina Burgos da Google Scholar
Thornley, Thomas Google Scholar
Pacheco-Silva, Alvaro Autor UNIFESP Google Scholar
Câmara, Niels Olsen Saraiva Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Albert Einstein Hosp
Harvard Univ
Abstract Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Brazilian Council of Scientific and Technologic Development
Complex Fluids INCT, Coordination of Improvement of Higher Education Personnel
Grant number FAPESP: 07/07139-3
FAPESP: 06/55326-4
FAPESP: 10/52180-4
FAPESP: 12/02270-2
Brazilian Council of Scientific and Technologic Development: 470533/2007-2
Brazilian Council of Scientific and Technologic Development: CNPq/DECIT/MS
Complex Fluids INCT, Coordination of Improvement of Higher Education Personnel: CAPES Bex-2236/09-5
Date 2013-10-04
Published in Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 9
Origin http://dx.doi.org/10.1371/journal.pone.0076396
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000325489100114
URI http://repositorio.unifesp.br/handle/11600/36868

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