Compartmentalization of pro-inflammatory cytokine levels in renal-transplanted pregnant women

Compartmentalization of pro-inflammatory cytokine levels in renal-transplanted pregnant women

Author Correa-Silva, Simone Google Scholar
Prado, Karen Matias do Google Scholar
Oliveira, Leandro Gustavo Autor UNIFESP Google Scholar
Ono, Erica Autor UNIFESP Google Scholar
Saraiva Camara, Niels Olsen Google Scholar
Bevilacqua, Estela Google Scholar
Institution Universidade de São Paulo (USP)
UNESP São Paulo State Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: We evaluated whether chronic exposure to immunosuppression in transplant recipients modulate the placental inflammatory cytokine levels associated to gestational tolerance mechanisms.Methods: Serum samples were collected from 12 renal transplanted pregnant under immunosuppressive regimen treatment and 10 healthy women in second/third trimester of gestation. Term placental tissues (decidua and chorionic villi) were also obtained after elective caesarean. Serum IL-1 beta, IL-6, IL-8, IL-12p70 and TNF-alpha were measured, as also in placental homogenates, by Cytometric Bead Array (CBA) combined with flow cytometry and, TGF-beta and IL-18 were measured by ELISA.Results: Serum levels of IL-6 (p = 0.0001) and TNF-alpha (0.0112) were higher in the 2nd and 3rd trimesters and in decidua the spectrum of increased pro inflammatory cytokines was wider: IL-1 beta (p = 0.0001), IL-6 (p = 0.0001), IL-8 (p = 0.0001), IL-12p70 (p = 0.0001), TGF-beta (p = 0.0089) and TNF-alpha (p = 0.0002). TGF-beta 1 was particularly increased in decidual compartment (p = 0.001). in the chorionic villous, pro inflammatory profile also were maintained. High IL-1 beta (p = 0.0001), IL-6 (p = 0.0001), IL-8 (p = 0.0001) and TNF-alpha (p = 0.0001) levels establish a similar pattern to that seem in decidua.Conclusion: Immunosuppressors may impair the immune response, but when associated with pregnancy the cytokine levels seems to shift a proinflammatory profile in placental compartments, which might also impact on the gestational outcomes in transplanted mothers.
Keywords Cytokines
immune response
immunosuppressive drugs
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-10-01
Published in Journal of Maternal-fetal & Neonatal Medicine. London: Informa Healthcare, v. 26, n. 15, p. 1468-1473, 2013.
ISSN 1476-7058 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 1468-1473
Access rights Closed access
Type Article
Web of Science ID WOS:000324588200002

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