Sialoglycoproteins in Morphological Distinct Stages of Mucor polymorphosporus and their Influence on Phagocytosis by Human Blood Phagocytes

Sialoglycoproteins in Morphological Distinct Stages of Mucor polymorphosporus and their Influence on Phagocytosis by Human Blood Phagocytes

Author Almeida, Catia Amancio Google Scholar
Campos-Takaki, Galba Maria de Google Scholar
Portela, Maristela Barbosa Google Scholar
Travassos, Luiz R. Autor UNIFESP Google Scholar
Alviano, Celuta Sales Google Scholar
Alviano, Daniela Sales Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Univ Catolica Pernambuco
Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Abstract The possible role of sialic acids in host cells-fungi interaction and their association with glycoproteins were evaluated using a clinical isolate of the dimorphic fungus Mucor polymorphosporus. Lectin-binding assays with spores and yeast cells denoted the presence of surface sialoglycoconjugates containing 2,3- and 2,6-linked sialylglycosyl groups. Western blotting with peroxidase-labeled Limulus polyphemus agglutinin revealed the occurrence of different sialoglycoprotein types in both cell lysates and cell wall protein extracts of mycelia, spores, and yeasts of M. polymorphosporus. Sialic acids contributed to the surface negative charge of spores and yeast forms as evaluated by adherence to a cationic substrate. Sialidase-treated spores were less resistant to phagocytosis by human neutrophils and monocytes from healthy individuals than control (untreated) fungal suspensions. the results suggest that sialic acids are terminal units of various glycoproteins of M. polymorphosporus, contributing to negative charge of yeasts and spore cells and protecting infectious propagules from destruction by host cells.
Keywords Sialoglycoproteins
Mucor polymorphosporus
Mucor spp.
Mycelium
Spore
Yeast
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2013-10-01
Published in Mycopathologia. Dordrecht: Springer, v. 176, n. 3-4, p. 183-189, 2013.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 183-189
Origin http://dx.doi.org/10.1007/s11046-013-9692-6
Access rights Closed access
Type Article
Web of Science ID WOS:000325135500002
URI http://repositorio.unifesp.br/handle/11600/36802

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