Identification of upregulated genes in oral squamous cell carcinomas

Identification of upregulated genes in oral squamous cell carcinomas

Author Lessa, Roberta C. Google Scholar
Campos, Antonio Hugo J. F. M. Google Scholar
Freitas, Carlos Elias de Google Scholar
Silva, Felipe Rodrigues da Google Scholar
Kowalski, Luiz Paulo Google Scholar
Carvalho, Andre Lopes Google Scholar
Vettore, Andre Luiz Autor UNIFESP Google Scholar
Institution AC Camargo Canc Hosp
Ludwig Inst Canc Res
Bahia Sch Med & Publ Hlth
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Barretos Canc Hosp
Universidade Federal de São Paulo (UNIFESP)
Abstract BackgroundOral cancer is the most common subset of head and neck squamous cell carcinomas (HNSCC). These tumors often have an aggressive clinical outcome hallmarked by a propensity for local invasion and regional nodal metastasis. Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for these tumors.MethodsTo identify upregulated genes in oral squamous cell carcinomas (OSSCs), we examined the ORESTES public database and used a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) approach to determine the expression level of selected genes in tumor samples.Results and ConclusionsThe ORESTES data mining analysis indicated 40 upregulated genes in HNSCC. Nine of these candidate genes were selected for further qRT-PCR validation and 3 of them (ALDOA, AHSA1, and POLQ) were frequently found upregulated in OSCC samples, which may indicate an association of these genes with the carcinogenesis process in this tumor site and they can constitute potential new targets for therapy. (c) 2012 Wiley Periodicals, Inc. Head Neck 35: 1475-1481, 2013
Keywords head and neck squamous cell carcinoma
upregulation
quantitative RT-PCR
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundacao Antonio Prudente (FAP)
Grant number CNPq: 476586/2006-2
FAPESP: 05/02580-8
CNPq: 302360/2008-5
CNPq: 308732/2011-1
CNPq: 313181/2009-8
Date 2013-10-01
Published in Head and Neck-Journal for the Sciences and Specialties of the Head and Neck. Hoboken: Wiley-Blackwell, v. 35, n. 10, p. 1475-1481, 2013.
ISSN 1043-3074 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1475-1481
Origin http://dx.doi.org/10.1002/hed.23169
Access rights Closed access
Type Article
Web of Science ID WOS:000325091000028
URI http://repositorio.unifesp.br/handle/11600/36789

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