Effect of exhaustive ultra-endurance exercise in muscular glycogen and both Alpha1 and Alpha2 Ampk protein expression in trained rats

Effect of exhaustive ultra-endurance exercise in muscular glycogen and both Alpha1 and Alpha2 Ampk protein expression in trained rats

Author Tarini, V. A. F. Autor UNIFESP Google Scholar
Carnevali, L. C. Google Scholar
Arida, R. M. Autor UNIFESP Google Scholar
Cunha, C. A. Autor UNIFESP Google Scholar
Alves, E. S. Autor UNIFESP Google Scholar
Seeleander, M. C. L. Google Scholar
Schmidt, B. Autor UNIFESP Google Scholar
Faloppa, F. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Glycogen is the main store of readily energy in skeletal muscle and plays a key role in muscle function, demonstrated by the inability to sustain prolonged high-intensity exercise upon depletion of these glycogen stores. With prolonged exercise, glycogen depletion occurs and 5'-AMP-activated protein kinase (AMPK), a potent regulator of muscle metabolism and gene expression, is activated promoting molecular signalling that increases glucose uptake by muscular skeletal cells. the aim of this study was primarily to determine the effect of ultra-endurance exercise on muscle glycogen reserves and secondly to verify the influence of this type of exercise on AMPK protein expression. Twenty-four male Wistar rats, 60 days old, were divided into four experimental groups: sedentary, sedentary exhausted (SE), endurance trained (T) and endurance trained exhausted (TE). the animals ran for 10 to 90 min/day, 5 days/week, for 12 weeks to attain trained status. Rats were killed immediately after the exhaustion protocol, which consisted of running on a treadmill (at approximately 60 % V (max) until exhaustion). Optical density of periodic acid-Schiff was detected and glycogen depletion observed predominantly in type I muscle fibres of the TE group and in both type I and II muscle fibres in the SE group. Plasma glucose decreased only in the TE group. Hepatic glycogen was increased in T group and significantly depleted in TE group. AMPK protein expression was significantly elevated in TE and T groups. in conclusion, acute exhaustive ultra-endurance exercise promoted muscle glycogen depletion. It seems that total AMPK protein and gene expression is more influenced by status training.
Keywords Ultra-endurance
Exhaustion
Glycogen
Language English
Date 2013-09-01
Published in Journal of Physiology and Biochemistry. Pamplona: Servicio Publicaciones Universidad Navarra, v. 69, n. 3, p. 429-440, 2013.
ISSN 1138-7548 (Sherpa/Romeo, impact factor)
Publisher Servicio Publicaciones Universidad Navarra
Extent 429-440
Origin http://dx.doi.org/10.1007/s13105-012-0224-5
Access rights Closed access
Type Article
Web of Science ID WOS:000322730300008
URI http://repositorio.unifesp.br/handle/11600/36705

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