BONE MARROW STEM CELLS in FACIAL NERVE REGENERATION FROM ISOLATED STUMPS

BONE MARROW STEM CELLS in FACIAL NERVE REGENERATION FROM ISOLATED STUMPS

Author Salomone, Raquel Google Scholar
Bento, Ricardo F. Google Scholar
Costa, Heloisa J. Z. R. Google Scholar
Azzi-Nogueira, Deborah Google Scholar
Ovando, Patricia C. Autor UNIFESP Google Scholar
Da-Silva, Cira F. Google Scholar
Zanatta, Daniela B. Google Scholar
Strauss, Bryan E. Google Scholar
Haddad, Luciana A. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract IntroductionSevere lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods: We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results: Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results.
Keywords electromyography
facial nerve
nerve conduction
nerve regeneration
Schwann cells
stem cells
Language English
Sponsor INCT Program Project of the National Council for Scientific and Technological Development
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 573633/2008-8
FAPESP: CEPID 1998/14254-2
FAPESP: 2008/53857-8
FAPESP: 2008/00458-9
Date 2013-09-01
Published in Muscle & Nerve. Hoboken: Wiley-Blackwell, v. 48, n. 3, p. 423-429, 2013.
ISSN 0148-639X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 423-429
Origin http://dx.doi.org/10.1002/mus.23768
Access rights Closed access
Type Article
Web of Science ID WOS:000327815800016
URI http://repositorio.unifesp.br/handle/11600/36689

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