Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples

Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples

Author Morais Sana, Dandara Emery Google Scholar
Miranda, Priscila Mayrink de Google Scholar
Vieira Pitol, Bruna Caroline Google Scholar
Moran, Mariana Soares Google Scholar
Toledo Silva, Nayara Nascimento Google Scholar
Cotrim Guerreiro da Silva, Ismael Dali Autor UNIFESP Google Scholar
Stocco, Rita de Cassia Google Scholar
Becak, Willy Google Scholar
Lima, Angelica Alves Google Scholar
Carneiro, Claudia Martins Google Scholar
Institution Univ Fed Ouro Preto
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract Herein, we evaluated cervical samples from normal tissue or HPV-infected tissue, to determine if the relative nuclear/cytoplasmic ratio (NA/CA) and the presence of nonclassical cytological criteria are a novel cytological criterion for the diagnosis of HPV. Significantly, larger NA/CA ratios were found for the HPV-ATYPIA+ and HPV+ATYPIA+ groups compared with HPV-ATYPIA- group, regardless of collection method. for the samples collected with a spatula, only three samples from the HPV-ATIPIA- group showed four or more nonclassical parameters (i.e., were positive), while a larger number of the samples in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups were positive (13, 4, and 13 samples, respectively). Among those collected with a brush, no sample showed four or more nonclassical criteria in the HPV-ATYPIA- group, while a number of samples were positive in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups (4, 3, and 4 samples, respectively). HPV infection was associated with significant morphometrical changes; no increase in the NA/CA ratio was found in the HPV+ATYPIA- samples, compared with the HPV-ATIPIA- samples collected with either a spatula or a brush. in conclusion, by including nonclassical cytological criteria into the patient diagnosis, we were able to reduce the number of false negative and false positive HPV diagnoses made using conventional cytology alone. Diagn. Cytopathol. 2013;41:785-792. (c) 2013 Wiley Periodicals, Inc.
Keywords HPV
cervix-vaginal cytology
nonclassical criteria
molecular biology
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Universidade Federal de Ouro Preto, Brazil
Date 2013-09-01
Published in Diagnostic Cytopathology. Hoboken: Wiley-Blackwell, v. 41, n. 9, p. 785-792, 2013.
ISSN 8755-1039 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 785-792
Access rights Closed access
Type Article
Web of Science ID WOS:000323410600006

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