The Value of Specific IgE to Peanut and Its Component Ara h 2 in the Diagnosis of Peanut Allergy

The Value of Specific IgE to Peanut and Its Component Ara h 2 in the Diagnosis of Peanut Allergy

Author Lopes de Oliveira, Lucila C. Autor UNIFESP Google Scholar
Aderhold, Martina Google Scholar
Brill, Marianne Google Scholar
Schulz, Gabriele Google Scholar
Rolinck-Werninghaus, Claudia Google Scholar
Mills, E. N. Clare Google Scholar
Niggemann, Bodo Google Scholar
Naspitz, Charles Kirov Autor UNIFESP Google Scholar
Wahn, Ulrich Google Scholar
Beyer, Kirsten Google Scholar
Institution Charite
Universidade Federal de São Paulo (UNIFESP)
VBC Genom
Inst Food Res
German Red Cross Hosp Westend
Abstract BACKGROUND: To avoid unnecessary oral food challenges, which are time consuming, stressful, and risky, improved in vitro diagnostic methods for food allergy such as component resolved diagnostics are still under investigation.OBJECTIVE: To investigate the role of whole peanut-and peanut-component (Ara h 1, Ara h 2, Ara h 3, Ara h 6 and Ara h 8)-specific IgE levels in the diagnostic procedure of peanut allergy as well as the diagnostic properties of peanut-specific IgG and IgG(4).METHODS: Sixty-one children underwent oral peanut challenge tests for diagnostic purposes irrespective of their peanut-specific IgE levels. Peanut-specific serum IgE, IgG, and IgG(4) levels were determined by ImmunoCAP FEIA and specific IgE against individual peanut proteins by Immuno Solid-phase Allergen Chip.RESULTS: Thirty-four of 61 patients (56%) had a peanut allergy. No significant difference was observed for peanut-specific IgG or peanut-specific IgG(4) levels between patients who were allergic and tolerant patients, whereas peanut-specific IgE was significant higher in patients who were allergic than in tolerant patients (P < .005). Twenty-five of 61 children had peanut-specific IgE above a previously proposed cutoff level of 15 kU(A)/L; however, 7 of these 25 children (28%) were clinically tolerant. Ara h 2-specific IgE was significantly lower in tolerant than in patients with allergies (P < .0001). Interestingly, 94% of the patients with peanut allergies showed IgE-binding to Ara h 2. Unfortunately, 26% of the sensitized but tolerant patients have shown IgE-binding to Ara h 2 too.CONCLUSIONS: Neither the level of specific IgE to peanut nor to Ara h 2 was able to clearly distinguish patients with clinical relevant peanut allergy from those who were clinical tolerant in our population. As expected, peanut-specific IgG and IgG(4) did not improve the diagnostic procedure. (C) 2013 American Academy of Allergy, Asthma & Immunology
Keywords IgE
IgG
IgG(4)
Peanut allergy
Immuno Solid-phase Allergen Chip
Component-resolved diagnostics
Ara h 1
Ara h 2
Ara h 3
Ara h 6
Ara h 8
Language English
Sponsor European Commission
German Research Foundation
Grant number European Commission: FOOD-CT-2005-514000
German Research Foundation: DFG-BE3991/1-1
Date 2013-07-01
Published in Journal of Allergy and Clinical Immunology-in Practice. Amsterdam: Elsevier B.V., v. 1, n. 4, p. 394-398, 2013.
ISSN 2213-2198 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 394-398
Origin http://dx.doi.org/10.1016/j.jaip.2013.05.010
Access rights Closed access
Type Article
Web of Science ID WOS:000209374500011
URI http://repositorio.unifesp.br/handle/11600/36489

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