Gradual strenuous running regimen predisposes to osteoarthritis due to cartilage cell death and altered levels of glycosaminoglycans

Gradual strenuous running regimen predisposes to osteoarthritis due to cartilage cell death and altered levels of glycosaminoglycans

Author Franciozi, Carlos Eduardo da Silveira Autor UNIFESP Google Scholar
Tarini, Victor Alexandre Ferreira Autor UNIFESP Google Scholar
Reginato, Rejane Daniele Autor UNIFESP Google Scholar
Gonçalves, Patrícia dos Reis Sousa Autor UNIFESP Google Scholar
Medeiros, Valquíria Pereira de Autor UNIFESP Google Scholar
Ferretti, Mario Autor UNIFESP Google Scholar
Dreyfuss, Juliana Luporini Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Faloppa, Flávio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: To investigate the hypothesis that strenuous running is a predisposing factor for osteoarthritis.Design: Wistar rats were divided into two groups: a control group (CG) and a trained group (TG). the TG underwent a strenuous treadmill running training regimen of controlled intensity, exhibiting progressively improvement of fitness over 12 weeks, running at least 55 km during this period and finally performing an ultra-endurance running exercise to exhaustion. After this period, rats from both groups were euthanized and their knees removed. the articular cartilage was dissected and submitted to histomorphometrical, histomorphological, and immunohistochemical analyses evaluating cell death pathway (caspase-3 and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick 'end labeling (TUNEL)) and inflammatory cytokines [interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor-alpha (TNF-alpha)]. in addition, the tissues were analyzed regarding the types and the content of glycosaminoglycans.Results: the TG knee joints exhibited increase in the number of chondrocytes and chondrocyte clusters, as well as significantly increased levels of caspase-3, a protein involved in apoptosis, and of inflammatory cytokines IL-1 alpha and TNF-alpha. in addition, histologically higher grades of osteoarthritis (Osteoarthritis Research Society International - OARSI grading), and significantly decreased levels of chondroitin sulfate and hyaluronic acid. Knee cartilage thickness and TUNEL did not significantly differ between the two groups.Conclusions: the articular cartilage of rats subjected to a strenuous running regimen of controlled intensity exhibited molecular and histological characteristics that are present in osteoarthritis. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier B.V. All rights reserved.
Keywords Articular cartilage
Extracellular matrix
Apoptosis
Running
Chondroitin sulfate
Hyaluronic acid
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-07-01
Published in Osteoarthritis and Cartilage. Oxford: Elsevier B.V., v. 21, n. 7, p. 965-972, 2013.
ISSN 1063-4584 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 965-972
Origin http://dx.doi.org/10.1016/j.joca.2013.04.007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000321882900012
URI http://repositorio.unifesp.br/handle/11600/36443

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