Establishment and cytogenetic characterization of a cell line from a pulmonary metastasis of osteosarcoma

Establishment and cytogenetic characterization of a cell line from a pulmonary metastasis of osteosarcoma

Author Salinas-Souza, Carolina Autor UNIFESP Google Scholar
Oliveira, Indhira Dias Autor UNIFESP Google Scholar
Oliveira, Renato de Autor UNIFESP Google Scholar
Seixas Alves, Maria Teresa de Autor UNIFESP Google Scholar
Petrilli, Antonio Sergio Autor UNIFESP Google Scholar
Toledo, Silvia Regina Caminada de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Osteosarcoma (OS) is the most frequent malignant bone tumour in children and adolescents. in metastatic patients, the most common site of metastasis is the lung. There are relatively few cell lines of metastatic OS reported in the literature and the cytogenetic aspects of OS metastases are still controversial and inconclusive. Here we describe the establishment of a new OS cell line, M-OS, from a pulmonary metastasis of a typical osteoblastic OS of an 11-year-old boy with metastatic OS at diagnosis. M-OS cells have been maintained in culture for over 50 passages for more than 1 year. M-OS was characterized by immunohistochemistry, conventional cytogenetics and fluorescence in situ hybridization (FISH). in order to evaluate in vitro cell modification, the immunohistochemical analysis was performed in three different moments of the cell line: 10th, 30th and 50th passages. the conventional cytogenetic analysis revealed the ploidy of M-OS cell line as near-diploid, with most metaphases hyperdiploid and tetraploid. We found a copy number gain of MDM2 gene as the most frequent alteration in the FISH analysis. the immunohistochemical analysis confirmed that M-OS cell line maintained the osteogenic nature even after all passages for the cell line establishment in vitro.
Keywords Osteosarcoma
Lung metastasis
Cell line
Cytogenetics
FISH
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grupo de Apoio ao Adolescente e a Crianca com Cancer (GRAACC/UNIFESP)
Date 2013-05-01
Published in Cytotechnology. Dordrecht: Springer, v. 65, n. 3, p. 347-353, 2013.
ISSN 0920-9069 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 347-353
Origin http://dx.doi.org/10.1007/s10616-012-9487-5
Access rights Closed access
Type Article
Web of Science ID WOS:000316220500004
URI http://repositorio.unifesp.br/handle/11600/36278

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