Metagenomic Analysis of a Tropical Composting Operation at the São Paulo Zoo Park Reveals Diversity of Biomass Degradation Functions and Organisms

Metagenomic Analysis of a Tropical Composting Operation at the São Paulo Zoo Park Reveals Diversity of Biomass Degradation Functions and Organisms

Author Martins, Layla Farage Google Scholar
Antunes, Luciana Principal Google Scholar
Pascon, Renata C. Autor UNIFESP Google Scholar
Oliveira, Julio Cezar Franco de Autor UNIFESP Google Scholar
Digiampietri, Luciano A. Google Scholar
Barbosa, Deibs Google Scholar
Peixoto, Bruno Malveira Google Scholar
Vallim, Marcelo Afonso Autor UNIFESP Google Scholar
Viana-Niero, Cristina Autor UNIFESP Google Scholar
Ostroski, Eric H. Google Scholar
Telles, Guilherme P. Google Scholar
Dias, Zanoni Google Scholar
Cruz, Joao Batista da Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Verjovski-Almeida, Sergio Google Scholar
Silva, Aline Maria da Google Scholar
Setubal, Joao Carlos Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Fundacao Parque Zool São Paulo
Virginia Tech
Abstract Composting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the São Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. the phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo compost metagenomes compared with seven selected public metagenomes of biomass deconstruction environments, indicating the potential for different bacterial communities to provide alternative mechanisms for the same functional purposes. Our results indicate that biomass degradation in this composting process, including deconstruction of recalcitrant lignocellulose, is fully performed by bacterial enzymes, most likely by members of the Clostridiales and Actinomycetales orders.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2009/52030-5R
Date 2013-04-24
Published in Plos One. San Francisco: Public Library Science, v. 8, n. 4, 13 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 13
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000318340400058

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