Effects of Light Exposure, pH, Osmolarity, and Solvent on the Retinal Pigment Epithelial Toxicity of Vital Dyes

Effects of Light Exposure, pH, Osmolarity, and Solvent on the Retinal Pigment Epithelial Toxicity of Vital Dyes

Author Costa, Elaine F. Autor UNIFESP Google Scholar
Barros, Nilana M. T. Autor UNIFESP Google Scholar
Coppini, Larissa Pereira Autor UNIFESP Google Scholar
Neves, Raquel L. Autor UNIFESP Google Scholar
Carmona, Adriana K. Autor UNIFESP Google Scholar
Penha, Fernando M. Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Magalhaes, Octaviano Autor UNIFESP Google Scholar
Moraes-Filho, Milton N. Autor UNIFESP Google Scholar
Lima Filho, Acacio A. S. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Farah, Michel E. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To investigate the in vitro effect of pH, osmolarity, solvent, and light interaction on currently used an l novel dyes to minimize dye-related retinal toxicity.DESIGN: Laboratory investigation.METHODS: Retinal pigment epithelium (RPE) human cells (ARPE-19) were exposed for 10 minutes to different pH solutions (4, 5, 6, 7, 7.5, 8, and 9) and glucose solutions (2.5%, 5.0%, 10%, 20%, 40%, and 50%) with osmolarity from 142 to 2530 mOsm, with and without 0.5 mg/mL trypan blue. R28 cells were also incubated with glucose (150, 310, and 1000 mOsm) and mannitol used as an osmotic control agent in both experiments. Dye-light interaction was assessed by incubating ARPE-19 for 10 minutes with trypan blue, brilliant blue, bromophenol blue, fast green, light green, or indigo carmine (0.05 mg/mL diluted in balanced saline solution) in the presence of high-brightness xenon and mercury vapor light sources.RESULTS: Solutions with nonphysiologic pH, below 7 and above 7.5, proved to be remarkably toxic to RPE cells with or without trypan blue. Also, all glucose solutions were deleterious to RPE (P < .001) even in iso-osmolar range. No harmful effect was found with mannitol solutions. Among the dyes tested, only light green and fast green were toxic to ARPE-19 (P < .001). Light exposure did not increase RPE toxicity either with xenon light or mercury vapor lamp.CONCLUSIONS: Solutions containing glucose as a dye solvent or nonphysiologic pH should be used with care in surgical situations where the RPE is exposed. Light exposure under present assay conditions did not increase the RPE toxicity. (Am J Ophthalmol 2013;155:705-712. (C) 2013 by Elsevier Inc. All rights reserved.)
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Pan-American Association of Ophthalmology/Pan-American Ophthalmological Foundation (PAAO/PAOF; Arlington, Texas)
Date 2013-04-01
Published in American Journal of Ophthalmology. New York: Elsevier B.V., v. 155, n. 4, p. 705-712, 2013.
ISSN 0002-9394 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 705-712
Origin http://dx.doi.org/10.1016/j.ajo.2012.10.004
Access rights Closed access
Type Article
Web of Science ID WOS:000317022400013
URI http://repositorio.unifesp.br/handle/11600/36153

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