DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis induces long-term protection in presence of regulatory T cells

Show simple item record

dc.contributor.author Amorim, Juliana de
dc.contributor.author Magalhães, Adriana
dc.contributor.author Munoz, Julian Esteban
dc.contributor.author Rittner, Glauce Mary Gomes
dc.contributor.author Nosanchuk, Joshua Daniel
dc.contributor.author Travassos, Luiz Rodolpho [UNIFESP]
dc.contributor.author Taborda, Carlos Pelleschi [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:17Z
dc.date.available 2016-01-24T14:31:17Z
dc.date.issued 2013-03-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2012.11.007
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 15, n. 3, p. 181-191, 2013.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35994
dc.description.abstract Paracoccidioidomycosis is a granulomatous systemic mycosis endemic in Brazil and other Latin America countries. A DNA vaccine encoding the inununoprotective peptide 10 (P10) significantly reduced the fungal burden in mice when given prior to or after intratracheal challenge with Paracoccidioides brasiliensis. Presently, the generation/expansion of CD4(+) CD44(hi) memory T cells as well as Foxp3(+) Treg cells in mice immunized with the DNA vaccine (pcDNA3-P10) before and after infection with P. brasiliensis was investigated. Memory CD4(+) CD44(hi) T cells simultaneously with Foxp3(+) Treg cells increased in the spleens and lungs of pcDNA3-P10 immunized mice on day 0, 30, 60 and 120 postinfection. Histopathology of the lung tissue showed minimal inflammation in immunized mice compared with the unimmunized group, suggesting a role for regulatory T cells in controlling the immunopathology. the DNA vaccine shows that the repeated immunization generates memory cells and regulatory T cells that replace the initially protective pro-inflammatory T cells conferring a long term protection while preserving the integrity of the infected tissue. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 181-191
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso aberto
dc.subject P10 peptide en
dc.subject Paracoccidioides brasiliensis en
dc.subject Immunization en
dc.subject Regulatory T cells en
dc.title DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis induces long-term protection in presence of regulatory T cells en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Albert Einstein Coll Med
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliation Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA
dc.description.affiliation Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Lab Med Mycol IMT SP LIM53, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 11/17267-4
dc.description.sponsorshipID FAPESP: 09/15823-7
dc.description.sponsorshipID FAPESP: 10/51423-0
dc.identifier.file WOS000317316100003.pdf
dc.identifier.doi 10.1016/j.micinf.2012.11.007
dc.description.source Web of Science
dc.identifier.wos WOS:000317316100003



File

Name: WOS000317316100003.pdf
Size: 1.057Mb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account