DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis induces long-term protection in presence of regulatory T cells

DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis induces long-term protection in presence of regulatory T cells

Author Amorim, Juliana de Google Scholar
Magalhães, Adriana Google Scholar
Munoz, Julian Esteban Google Scholar
Rittner, Glauce Mary Gomes Google Scholar
Nosanchuk, Joshua Daniel Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Taborda, Carlos Pelleschi Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Albert Einstein Coll Med
Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccidioidomycosis is a granulomatous systemic mycosis endemic in Brazil and other Latin America countries. A DNA vaccine encoding the inununoprotective peptide 10 (P10) significantly reduced the fungal burden in mice when given prior to or after intratracheal challenge with Paracoccidioides brasiliensis. Presently, the generation/expansion of CD4(+) CD44(hi) memory T cells as well as Foxp3(+) Treg cells in mice immunized with the DNA vaccine (pcDNA3-P10) before and after infection with P. brasiliensis was investigated. Memory CD4(+) CD44(hi) T cells simultaneously with Foxp3(+) Treg cells increased in the spleens and lungs of pcDNA3-P10 immunized mice on day 0, 30, 60 and 120 postinfection. Histopathology of the lung tissue showed minimal inflammation in immunized mice compared with the unimmunized group, suggesting a role for regulatory T cells in controlling the immunopathology. the DNA vaccine shows that the repeated immunization generates memory cells and regulatory T cells that replace the initially protective pro-inflammatory T cells conferring a long term protection while preserving the integrity of the infected tissue. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords P10 peptide
Paracoccidioides brasiliensis
Immunization
Regulatory T cells
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 11/17267-4
FAPESP: 09/15823-7
FAPESP: 10/51423-0
Date 2013-03-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 15, n. 3, p. 181-191, 2013.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 181-191
Origin http://dx.doi.org/10.1016/j.micinf.2012.11.007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000317316100003
URI http://repositorio.unifesp.br/handle/11600/35994

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