Chlorella vulgaris treatment ameliorates the suppressive effects of single and repeated stressors on hematopoiesis

Chlorella vulgaris treatment ameliorates the suppressive effects of single and repeated stressors on hematopoiesis

Author Queiroz, Julia de Souza Google Scholar
Barbosa, Christiano Marcello Vaz Autor UNIFESP Google Scholar
Rocha, Michelle C. da Google Scholar
Bincoletto, Claudia Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Souza Queiroz, Mary Luci de Souza Google Scholar
Palermo Neto, João Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The reports regarding the mutual influence between the central nervous system and the immune system constitute a vast and somewhat controversial body of literature. Stress is known to disturb homeostasis, impairing immunological functions. in this study, we investigated the hematopoietic response of Chlorella vulgaris (CV)-treated mice exposed to single (SST) and repeated stress (RST). We observed a reduction in the numbers of hematopoietic progenitors (HP) in the bone marrow and long-term bone marrow cultures (LTBMC) using flow cytometry and a coinciding decrease in the number of granulocyte-macrophage colonies (CFU-GM) after treatment with both stressors, but SST caused a more profound suppression. We observed a proportional increase in the colony-stimulating activity (CSA) of the serum of animals subjected to SST or RST. in the bone marrow, SST and RST induced a decrease in both mature myeloid and lymphoid populations but did not affect pluripotent hematopoietic progenitors (Lin(-)Sca-1(+)c-kit(+), LSK), and again, a more profound suppression was observed after SST. We further quantified the levels of interleukin-1 alpha (IL-1 alpha) and interleukin-6 (IL-6) and the number of myeloid cells in LTBMC. Both SST and RST reduced the levels of these cytokines to similar degrees. the myeloid population was also reduced in LTBMC, and SST induced a more intense suppression. Importantly, CV treatment prevented the changes produced by SST and RST in all of the parameters evaluated. Together, our results suggest that CV treatment is an effective tool for the prophylaxis of myelosuppression caused by single or repeated stressors. (c) 2012 Elsevier Inc. All rights reserved.
Keywords Chlorella vulgaris
Single stressor
Repeated stressor
Hematopoiesis
Flow cytometry
Long-term bone marrow culture
CFU-GM
Cytokines
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 09/51886-3
CNPq: 300764/2010-3
Date 2013-03-01
Published in Brain Behavior and Immunity. San Diego: Academic Press Inc Elsevier Science, v. 29, p. 39-50, 2013.
ISSN 0889-1591 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 39-50
Origin http://dx.doi.org/10.1016/j.bbi.2012.12.001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000315365400005
URI http://repositorio.unifesp.br/handle/11600/35990

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