Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

Author Cordeiro, Rossana de Aguiar Google Scholar
Nobre Patoilo, Kharla Rabelo Google Scholar
Praciano, Silviane Bandeira Google Scholar
Astete Medrano, Delia Jessica Google Scholar
Farias Marques, Francisca Jakelyne de Google Scholar
Soares Martins, Liline Maria Google Scholar
Eulalio, Kelsen Dantas Google Scholar
Deus Filho, Antonio de Google Scholar
Salmito Cavalvanti, Maria do Amparo Google Scholar
Bezerra Fechine, Maria Auxiliadora Google Scholar
Nogueira Brilhante, Raimunda Samia Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Gadelha Rocha, Marcos Fabio Google Scholar
Costa Sidrim, Jose Julio Google Scholar
Institution Univ Fed Ceara
Univ Fed Piaui
Inst Doencas Tropicais Natan Portella
Univ Integracao Int Lusofonia Afrobrasileira
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Ceara
Abstract Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. in this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. the highest reactivity in the ID tests was seen in the F0-90 antigen. in the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. in conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. the tests could improve serological diagnosis of coccidioidomycosis in South America.
Keywords Coccidioidomycosis
Enzyme immunoassay
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
National Council for Scientific and Technological Development (PRONEX)
Grant number CNPq: 306637/2010-3
National Council for Scientific and Technological Development (PRONEX): 2155-6
Date 2013-02-01
Published in Mycopathologia. Dordrecht: Springer, v. 175, n. 1-2, p. 25-32, 2013.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 25-32
Access rights Closed access
Type Article
Web of Science ID WOS:000313968100003

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