CNP and DPYSL2 mRNA Expression and Promoter Methylation Levels in Brain of Alzheimer's Disease Patients

CNP and DPYSL2 mRNA Expression and Promoter Methylation Levels in Brain of Alzheimer's Disease Patients

Author Silva, Patricia Natalia Autor UNIFESP Google Scholar
Furuya, Tatiane Katsue Autor UNIFESP Google Scholar
Braga, Ianna Sampaio Autor UNIFESP Google Scholar
Rasmussen, Lucas Trevizani Google Scholar
Labio, Roger Willian de Google Scholar
Bertolucci, Paulo Henrique Autor UNIFESP Google Scholar
Chen, Elizabeth Suchi Autor UNIFESP Google Scholar
Turecki, Gustavo Google Scholar
Mechawar, Naguib Google Scholar
Payao, Spencer Luiz Autor UNIFESP Google Scholar
Mill, Jonathan Google Scholar
Smith, Marilia Cardoso Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fac Med Marilia FAMEMA
McGill Univ
Kings Coll London
Abstract Alzheimer's disease (AD) is a highly prevalent type of dementia in the elderly population. AD is a complex neurodegenerative disorder. Thus, epigenetic mechanisms that regulate gene expression might have an important role in AD. CNP (2',3'-Cyclic Nucleotide 3' Phosphodiesterase) gene encodes a protein used as an index of myelin alterations. DPYSL2 (Dihydropyrimidinase-like 2) is described as acting in structural and regulatory processes in the central nervous system, such as neural differentiation, neurotransmitter release, and stabilization of microtubules. in this study, we evaluated gene expression and epigenetic regulation of CNP and DPYSL2 genes in three postmortem brain regions (entorhinal and auditory cortices and hippocampus) of AD patients and healthy elderly controls. mRNA quantification was performed using qRT-PCR, and promoter DNA methylation patterns were determined by mass spectrometry using the Sequenom EpiTYPER platform. We observed CNP mRNA downregulation in entorhinal and auditory cortex in relation to the same regions of the control group. CNP alterations in the brain might suggest impairment in myelination leading to a synaptic and cognition loss. No AD-associated differences in CNP and DPYSL2 promoter DNA methylation were observed, suggesting that other mechanisms may be involved in mediating the observed CNP gene expression.
Keywords CNP
DPYSL2
epigenetics
gene expression
mRNA
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-01-01
Published in Journal of Alzheimers Disease. Amsterdam: Ios Press, v. 33, n. 2, p. 349-355, 2013.
ISSN 1387-2877 (Sherpa/Romeo, impact factor)
Publisher Ios Press
Extent 349-355
Origin http://dx.doi.org/10.3233/JAD-2012-121192
Access rights Closed access
Type Article
Web of Science ID WOS:000312598300008
URI http://repositorio.unifesp.br/handle/11600/35867

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