Effect of Vital Dyes on Retinal Pigmented Epithelial Cell Viability and Apoptosis: Implications for Chromovitrectomy

Effect of Vital Dyes on Retinal Pigmented Epithelial Cell Viability and Apoptosis: Implications for Chromovitrectomy

Author Penha, Fernando M. Autor UNIFESP Google Scholar
Pons, Marianne Google Scholar
Fiod Costa, Elaine de Paula Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Marin-Castano, Maria E. Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Int Chromovitrectomy Collaboration Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Miami
Abstract Purpose: To investigate the in vitro effect of vital dyes on toxicity and apoptosis in a human retinal pigment epithelial cell line. Methods: ARPE-19 cells were exposed to brilliant blue (BBG), Evans Blue (EB), bromophenol blue (BroB), indocyanine green (ICG), infracyanine green (IfCG), light green (LG), fast green (FG), indigo carmine (IC) and Congo red (CR). Balanced salt solution was used as the control. Five different concentrations and 2 exposure times were tested. Cell viability was determined by the MTS (1-solution methyl thiazolyl tetrazolium) assay and apoptosis by Bax expression on Western blot. Results: All dyes significantly reduced cell viability after 3 min of exposure at all concentrations (p < 0.01), except for BBG that was safe at concentrations up to 0.25 mg/ml and CR up to 0.05 mg/ml, while LG was safe at all concentrations. Toxicity was higher after 30 min of exposure. Expression of Bax was upregulated after all dye exposures, except BBG; ICG had the highest Bax expression (p < 0.01). Conclusions: Overall the safest dye was BBG followed by LG, IfCG, FG, CR, IC, BroB, EB and ICG. ICG was toxic at all concentrations and exposure times tested. Moreover, BBG was the only dye that did not induce apoptosis in ARPE-19 cells. (C) 2013 S. Karger AG, Basel
Keywords Apoptosis
Cell viability
Retinal pigment epithelium
Vital dyes
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
NIH center
Research to Prevent Blindness Unrestricted grant
Department of Defense (DOD)
Grant number NIH center: P30EY014801
Department of Defense (DOD): W81X-WH-09-1-0675
Date 2013-01-01
Published in Ophthalmologica. Basel: Karger, v. 230, p. 41-50, 2013.
ISSN 0030-3755 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 41-50
Origin http://dx.doi.org/10.1159/000354251
Access rights Closed access
Type Article
Web of Science ID WOS:000324444600007
URI http://repositorio.unifesp.br/handle/11600/35853

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