Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Staining Properties of Brilliant Blue Depending on Different Incubation Times and Solvents in Humans

Author Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Penha, Fernando M. Autor UNIFESP Google Scholar
Dib, Eduardo Autor UNIFESP Google Scholar
Melo, Gustavo B. Autor UNIFESP Google Scholar
Maia, Andre Autor UNIFESP Google Scholar
Farah, Michel E. Autor UNIFESP Google Scholar
Int Chromovitrectomy Collaboration Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: Intravitreous injection of vital dyes, e.g. brilliant blue (BBG), promotes better visualization of the internal limiting membrane (ILM). This paper investigates the staining properties of BBG depending on different incubation times and 2 types of solvents - 5% glucose (GL) or saline solution - in a prospective study in patients. Methods: This paper investigates various aspects of BBG in various methods. An interventional prospective study was conducted in patients to examine the binding properties of the blue dye diluted in either saline or 5% GL to epiretinal membranes (ERMs) and ILMs. Forty-nine consecutive patients older than 18 years scheduled for macular ERM, vitreomacular traction and macular hole surgeries were prospectively recruited. the primary outcomes of this study were the degree of ILM and ERM staining. the secondary outcomes of the study were the need of reinjection of BBG or any other dye, the ability of BBG to stain the vitreous, and frequency of complications. the staining of the ILM and ERM were graded as no staining, little, moderate or strong staining. Results:There was no correlation between age (p = 0.32) or gender (p = 0.33) in the staining affinity of BBG to either the ILM or ERM. BBG may be an appropriate staining agent for the ILM in the majority (82%) of surgeries; however, in approximately half of the cases (45%) surgeons considered BBG not enough for ERM coloring and visualization. There is a tendency of BBG to stain the ILM better when saline solution is used compared to GL 5%; however, this was not statistically significant (p = 0.64). There was no difference in the staining efficacy of BBG to the ERMs by either solution (p = 0.70), despite the low staining affinity. Conclusion: BBG became the state-of-the-art dye for ILM identification. Differences in staining properties may imply that BBG should not be considered as first-line stain for ERM surgery. BBG is effective in ILM staining in over 80% of macular hole surgeries. (C) 2013 S. Karger AG, Basel
Keywords Brilliant blue
Retina
Internal limiting membrane
Macular hole
Vitrectomy
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Pan-American Association of Ophthalmology (PAAO)
Date 2013-01-01
Published in Ophthalmologica. Basel: Karger, v. 230, p. 68-72, 2013.
ISSN 0030-3755 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 68-72
Origin http://dx.doi.org/10.1159/000353872
Access rights Closed access
Type Article
Web of Science ID WOS:000324444600010
URI http://repositorio.unifesp.br/handle/11600/35852

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