The validation of the Z-Scan technique for the determination of plasma glucose

The validation of the Z-Scan technique for the determination of plasma glucose

Author Alves, Sarah I. Autor UNIFESP Google Scholar
Silva, Elaine A. O. Autor UNIFESP Google Scholar
Costa, Simone S. Google Scholar
Sonego, Denise R. N. Autor UNIFESP Google Scholar
Hallack, Maira L. Autor UNIFESP Google Scholar
Coppini, Ornela L. Autor UNIFESP Google Scholar
Rowies, Fernanda Autor UNIFESP Google Scholar
Azzalis, Ligia A. Autor UNIFESP Google Scholar
Junqueira, Virginia B. C. Autor UNIFESP Google Scholar
Pereira, Edimar C. Autor UNIFESP Google Scholar
Rocha, Katya C. Autor UNIFESP Google Scholar
Fonseca, Fernando L. A. Autor UNIFESP Google Scholar
Costa, MFPCM Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Glucose is the main energy source for the human body. the concentration of blood glucose is regulated by several hormones including both antagonists: insulin and glucagon. the quantification of glucose in the blood is used for diagnosing metabolic disorders of carbohydrates, such as diabetes, idiopathic hypoglycemia and pancreatic diseases. Currently, the methodology used for this determination is the enzymatic colorimetric with spectrophotometric. This study aimed to validate the use of measurements of nonlinear optical properties of plasma glucose via the Z-Scan technique. for this we used samples of calibrator patterns that simulate commercial samples of patients (ELITech(C)). Besides calibrators, serum glucose levels within acceptable reference values (normal control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) and also overestimated (pathological control serum - Brazilian Society of Clinical Pathology and Laboratory Medicine) were used in the methodology proposal. Calibrator dilutions were performed and determined by the Z-Scan technique for the preparation of calibration curve. in conclusion, Z-Scan method can be used to determinate glucose levels in biological samples with enzymatic colorimetric reaction and also to apply the same quality control parameters used in biochemistry clinical.
Keywords Z-Scan
Nonlinear Optics
Biomedical Optics
Glucose
Calibration Curve
Language English
Date 2013-01-01
Published in 8th Iberoamerican Optics Meeting and 11th Latin American Meeting On Optics, Lasers, and Applications. Bellingham: Spie-int Soc Optical Engineering, v. 8785, 6 p., 2013.
ISSN 0277-786X (Sherpa/Romeo, impact factor)
Publisher Spie-int Soc Optical Engineering
Extent 6
Origin http://dx.doi.org/10.1117/12.2025994
Access rights Closed access
Type Conference paper
Web of Science ID WOS:000328616100016
URI http://repositorio.unifesp.br/handle/11600/35839

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