Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein

Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein

Author Ching Ching, Ana Tung Google Scholar
Favaro, Regiane Degan Google Scholar
Lima, Swiany Silveira Google Scholar
Muniz Chaves, Agtha de Alencar Google Scholar
Lima, Marcelo Andrade de Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Estima Abreu, Patricia A. Google Scholar
Ho, Paulo Lee Google Scholar
Institution Inst Butantan
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract LigB is an adhesin from pathogenic Leptospira that is able to bind to extracellular matrix and is considered a virulence factor. A shotgun phage display genomic library was constructed and used for panning against Heparan Sulfate Proteoglycan (HSPG). A phage clone encoding part of LigB protein was selected in panning experiments and showed specific binding to heparin. To validate the selected clone, fragments of LigB were produced as recombinant proteins and showed affinity to heparin and to mammalian cells. Heparin was also able to reduce the binding of rLB-Ct to mammalian cells. Our data suggests that the glycosaminoglycan moiety of the HSPG is responsible for its binding and could mediate the attachment of the recombinant protein rLB-Ct. Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue. (C) 2012 Elsevier Inc. All rights reserved.
Keywords Leptospira interrogans
Heparin
Phage display
LigB
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao Butantan
Date 2012-11-02
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 427, n. 4, p. 774-779, 2012.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 774-779
Origin http://dx.doi.org/10.1016/j.bbrc.2012.09.137
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000311134700016
URI http://repositorio.unifesp.br/handle/11600/35497

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