The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Author Montani, Daniela Antunes Autor UNIFESP Google Scholar
Cordeiro, Fernanda Bertuccez Autor UNIFESP Google Scholar
Regiani, Thais Autor UNIFESP Google Scholar
Victorino, Amanda Begati Autor UNIFESP Google Scholar
Pilau, Eduardo Jorge Google Scholar
Gozzo, Fabio Cesar Google Scholar
Ferreira, Christina Ramires Google Scholar
Fraietta, Renato Autor UNIFESP Google Scholar
Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Abstract This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy.Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built.S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the non-pregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation.We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.
Keywords Cumulus cell
Lipid profile
Pregnancy outcome
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2010/14732-5
Date 2012-11-01
Published in Journal of Assisted Reproduction and Genetics. New York: Springer/plenum Publishers, v. 29, n. 11, p. 1289-1297, 2012.
ISSN 1058-0468 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1289-1297
Access rights Closed access
Type Article
Web of Science ID WOS:000312071400019

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