Influence of sleep deprivation and morphine on the expression of inducible nitric oxide synthase and cyclooxygenase-2 in skin of hairless mice

Influence of sleep deprivation and morphine on the expression of inducible nitric oxide synthase and cyclooxygenase-2 in skin of hairless mice

Author Egydio, Flavia Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Noguti, Juliana Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Skin performs a host of primordial functions that keep the body alive. Morphine is a drug with immunosuppressant properties whose chronic use may lead to increased infection and delayed wound healing. Sleep is a fundamental biological phenomenon that promotes the integrity of several bodily functions. Sleep deprivation adversely affects several systems, particularly the immune system. the aim of this study was to perform an immunohistochemical evaluation on the expression of inducible nitric oxide synthase and cyclooxygenase-2 in skin of sleep-deprived mice and mice chronically treated with morphine. Adult hairless male mice were distributed into the following groups: Control, morphine, sleep-deprived, and morphine + SD. Morphine (10 mg/kg, subcutaneous) was injected every 12 h for 9 days. Morphine induced immunoexpression of cyclooxygenase-2 and nitric oxide synthase. Sleep deprivation did not modulate outcomes induced by morphine. Morphine, not sleep loss, induces cyclooxygenase-2 and nitric oxide synthase immunoexpression in the skin of hairless mice.
Keywords Sleep deprivation
morphine
wound healing
immune system
COX-2
iNOS
Language English
Sponsor Associacao Fundo de Incentivo a Pesquisa (AFIP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 98/14303-3
Date 2012-10-01
Published in Toxicology Mechanisms and Methods. London: Informa Healthcare, v. 22, n. 8, p. 577-583, 2012.
ISSN 1537-6516 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 577-583
Origin http://dx.doi.org/10.3109/15376516.2012.702797
Access rights Closed access
Type Article
Web of Science ID WOS:000308955000001
URI http://repositorio.unifesp.br/handle/11600/35404

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