TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction

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dc.contributor.author Andrade-Oliveira, Vinicius [UNIFESP]
dc.contributor.author Campos, Erika F. [UNIFESP]
dc.contributor.author Goncalves-Primo, Amador
dc.contributor.author Grenzi, Patricia C. [UNIFESP]
dc.contributor.author Medina-Pestana, Jose O. [UNIFESP]
dc.contributor.author Tedesco-Silva, Helio
dc.contributor.author Gerbase-DeLima, Maria [UNIFESP]
dc.date.accessioned 2016-01-24T14:27:43Z
dc.date.available 2016-01-24T14:27:43Z
dc.date.issued 2012-09-27
dc.identifier http://dx.doi.org/10.1097/TP.0b013e31825db680
dc.identifier.citation Transplantation. Philadelphia: Lippincott Williams & Wilkins, v. 94, n. 6, p. 589-595, 2012.
dc.identifier.issn 0041-1337
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/35287
dc.description.abstract Background. the participation of Toll-like receptor (TLR) 4, an innate immunity receptor, has been previously demonstrated in the pathogenesis of acute renal injury. We aimed to investigate whether messenger RNA (mRNA) levels of TLR4 and its adapter molecule, myeloid differentiation primary response gene (MYD) 88, are associated with delayed graft function (DGF) and could be used as biomarkers of its occurrence.Methods. TLR4 and MYD88 gene mRNA levels were evaluated with real-time polymerase chain reaction, in preimplantation biopsies (n=89) and first day posttransplantation samples of urine (n=67) and blood (n=80) from graft recipients and analyzed according to donor type (living or deceased) and DGF occurrence.Results. Expression levels of both genes were higher in biopsies from deceased donors than from living donors (P<0.001 for both) but did not differ between deceased-donor kidney transplants with and without DGF; in urine, TLR4 expression levels were higher in patients with prolonged DGF (DGF lasting 914 days) (P=0.05, compared with cases without DGF); in blood, lower mRNA levels of TLR4 and MYD88 predicted pDGF occurrence with an accuracy of 86% and 87%, respectively.Conclusion. the expression levels of TLR4 and MYD88 were higher in kidneys from deceased donors than from living donors. Lower levels of expression of both genes in blood were associated with DGF occurrence. the prediction of prolonged DGF by low TLR4 and MYD88 expression levels in blood with a greater the 85% accuracy was the most important finding of this study. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Associacao Fundo de Incentivo a Pesquisa
dc.format.extent 589-595
dc.language.iso eng
dc.publisher Lippincott Williams & Wilkins
dc.relation.ispartof Transplantation
dc.rights Acesso restrito
dc.subject Delayed graft function en
dc.subject TLR4 en
dc.subject Innate immunity en
dc.subject Kidney/renal transplantation en
dc.subject Ischemia-reperfusion en
dc.title TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction en
dc.type Artigo
dc.contributor.institution Assoc Fundo Incent Pesquisa
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Hosp Rim & Hipertensao
dc.description.affiliation Assoc Fundo Incent Pesquisa, Inst Imunogenet, BR-04040031 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliation Hosp Rim & Hipertensao, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2008/51327-1
dc.identifier.doi 10.1097/TP.0b013e31825db680
dc.description.source Web of Science
dc.identifier.wos WOS:000309223100012



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