TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction

TLR4 mRNA Levels as Tools to Estimate Risk for Early Posttransplantation Kidney Graft Dysfunction

Author Andrade-Oliveira, Vinicius Autor UNIFESP Google Scholar
Campos, Erika F. Autor UNIFESP Google Scholar
Goncalves-Primo, Amador Google Scholar
Grenzi, Patricia C. Autor UNIFESP Google Scholar
Medina-Pestana, Jose O. Autor UNIFESP Google Scholar
Tedesco-Silva, Helio Google Scholar
Gerbase-DeLima, Maria Autor UNIFESP Google Scholar
Institution Assoc Fundo Incent Pesquisa
Universidade Federal de São Paulo (UNIFESP)
Hosp Rim & Hipertensao
Abstract Background. the participation of Toll-like receptor (TLR) 4, an innate immunity receptor, has been previously demonstrated in the pathogenesis of acute renal injury. We aimed to investigate whether messenger RNA (mRNA) levels of TLR4 and its adapter molecule, myeloid differentiation primary response gene (MYD) 88, are associated with delayed graft function (DGF) and could be used as biomarkers of its occurrence.Methods. TLR4 and MYD88 gene mRNA levels were evaluated with real-time polymerase chain reaction, in preimplantation biopsies (n=89) and first day posttransplantation samples of urine (n=67) and blood (n=80) from graft recipients and analyzed according to donor type (living or deceased) and DGF occurrence.Results. Expression levels of both genes were higher in biopsies from deceased donors than from living donors (P<0.001 for both) but did not differ between deceased-donor kidney transplants with and without DGF; in urine, TLR4 expression levels were higher in patients with prolonged DGF (DGF lasting 914 days) (P=0.05, compared with cases without DGF); in blood, lower mRNA levels of TLR4 and MYD88 predicted pDGF occurrence with an accuracy of 86% and 87%, respectively.Conclusion. the expression levels of TLR4 and MYD88 were higher in kidneys from deceased donors than from living donors. Lower levels of expression of both genes in blood were associated with DGF occurrence. the prediction of prolonged DGF by low TLR4 and MYD88 expression levels in blood with a greater the 85% accuracy was the most important finding of this study.
Keywords Delayed graft function
Innate immunity
Kidney/renal transplantation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Associacao Fundo de Incentivo a Pesquisa
Grant number FAPESP: 2008/51327-1
Date 2012-09-27
Published in Transplantation. Philadelphia: Lippincott Williams & Wilkins, v. 94, n. 6, p. 589-595, 2012.
ISSN 0041-1337 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 589-595
Access rights Closed access
Type Article
Web of Science ID WOS:000309223100012

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