Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats

Experimental Model of Arthritis Induced by Paracoccidioides brasiliensis in Rats

Author Loth, Eduardo Alexandre Google Scholar
Biazin, Samia Khalil Google Scholar
Paula, Claudete Rodrigues Google Scholar
Garcia Simao, Rita de Cassia Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Puccia, Rosana Autor UNIFESP Google Scholar
Gandra, Rinaldo Ferreira Google Scholar
Institution Hosp Univ Oeste Parana
Universidade de São Paulo (USP)
Univ Estadual Oeste Parana
Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccidioidomycosis (PCM), a disease caused by the fungus Paracoccidioides brasiliensis (Pb), is highly prevalent in Brazil, where it is the principal cause of death by systemic mycoses. the disease primarily affects men aged 30-50 year old and usually starts as a pulmonary focus and then may spread to other organs and systems, including the joints. the present study aimed to develop an experimental model of paracoccidioidomycotic arthritis. Two-month-old male Wistar rats (n = 48) were used, divided in 6 groups: test groups EG/15 and EG/45 (received one dose of 100 mu l of saline containing 10(5) Pb viable yeasts in the knee); heat killed Pb-group HK/15 and HK/45 (received a suspension of 10(5) Pb nonviable yeasts in the knee) and control groups CG/15 and CG/45 (received only sterile saline in the knee). the rats were killed 15 and 45 days postinoculation. in contrast with the control rats, the histopathology of the joints of rats of the test groups (EG/15 and EG/45) revealed a picture of well-established PCM arthritis characterized by extensive sclerosing granulomatous inflammation with numerous multiple budding fungal cells. the X-ray examination revealed joint alterations in these groups. Only metabolic active fungi evoked inflammation. the experimental model was able to induce fungal arthritis in the knees of the rats infected with metabolic active P. brasiliensis. the disease tended to be regressive and restrained by the immune system. No evidence of fungal dissemination to the lungs was observed.
Keywords Paracoccidioidomycosis
Paracoccidioides brasiliensis
Virulence factors
Language English
Sponsor Fundacao Araucaria-Fundo Parana/SETI
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2012-09-01
Published in Mycopathologia. Dordrecht: Springer, v. 174, n. 3, p. 187-191, 2012.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 187-191
Origin http://dx.doi.org/10.1007/s11046-012-9537-8
Access rights Closed access
Type Article
Web of Science ID WOS:000306125600002
URI http://repositorio.unifesp.br/handle/11600/35211

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