Morphological and chemical changes in dentin after using endodontic agents: Fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study

Morphological and chemical changes in dentin after using endodontic agents: Fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study

Author Pascon, Fernanda Miori Google Scholar
Kantovitz, Kamila Rosamilia Google Scholar
Silva Soares, Luis Eduardo Google Scholar
Espirito Santo, Ana Maria do Autor UNIFESP Google Scholar
Martin, Airton Abrahao Google Scholar
Puppin-Rontani, Regina Maria Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
NIAMSD
Vale Paraiba Univ UNIVAP
Universidade Federal de São Paulo (UNIFESP)
Abstract We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (mu EDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n = 5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2% chlorhexidine + 17% EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25% NaOCl + 17% EDTA. the inorganic and organic content was analyzed by FT-Raman. mu EDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (p < 0.05). Differences were observed among groups for the 960 cm(-1) peak. Ca and P content differences were significant (SH5 > NT = SH5E > CHX > E > CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p < 0.05). the SEM images in the EDTA-treated groups had the highest number of open tubules. Erosion in the tubules was observed in CHX and SH5E groups. Endodontic agents change the inorganic and organic content of pulp chamber dentin. NaOCl used alone, or in association with EDTA, was the most effective agent considering chemical and morphological approaches. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). IDOL 10.1117/1.JBO.17.7.075008
Keywords dentin
endodontics
energy dispersive x-ray fluorescence spectrometry
pulp chamber
Raman spectroscopy
scanning electron microscopy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
São Paulo Research Support Foundation
Grant number São Paulo Research Support Foundation: 05/58561-1
São Paulo Research Support Foundation: 01/14384-8
São Paulo Research Support Foundation: 05/50811-9
Date 2012-07-01
Published in Journal of Biomedical Optics. Bellingham: Spie-soc Photo-optical Instrumentation Engineers, v. 17, n. 7, 6 p., 2012.
ISSN 1083-3668 (Sherpa/Romeo, impact factor)
Publisher Spie-soc Photo-optical Instrumentation Engineers
Extent 6
Origin http://dx.doi.org/10.1117/1.JBO.17.7.075008
Access rights Closed access
Type Article
Web of Science ID WOS:000307989500024
URI http://repositorio.unifesp.br/handle/11600/35078

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