Behavioral and genetic effects promoted by sleep deprivation in rats submitted to pilocarpine-induced status epilepticus

Behavioral and genetic effects promoted by sleep deprivation in rats submitted to pilocarpine-induced status epilepticus

Author Matos, Gabriela Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Alvarenga, Tathiana Aparecida Autor UNIFESP Google Scholar
Hirotsu, Camila Autor UNIFESP Google Scholar
Scorza, Fulvio Alexandre Autor UNIFESP Google Scholar
Le Sueur-Maluf, Luciana Autor UNIFESP Google Scholar
Noguti, Juliana Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The interaction between sleep deprivation and epilepsy has been well described in electrophysiological studies, but the mechanisms underlying this association remain unclear. the present study evaluated the effects of sleep deprivation on locomotor activity and genetic damage in the brains of rats treated with saline or pilocarpine-induced status epilepticus (SE). After 50 days of pilocarpine or saline treatment, both groups were assigned randomly to total sleep deprivation (TSD) for 6 h, paradoxical sleep deprivation (PSD) for 24 h, or be kept in their home cages. Locomotor activity was assessed with the open field test followed by resection of brain for quantification of genetic damage by the single cell gel electrophoresis (comet) assay. Status epilepticus induced significant hyperactivity in the open field test and caused genetic damage in the brain. Sleep deprivation procedures (TSD and PSD) did not affect locomotor activity in epileptic or healthy rats, but resulted in significant DNA damage in brain cells. Although PSD had this effect in both vehicle and epileptic groups. TSD caused DNA damage only in epileptic rats. in conclusion, our results revealed that, despite a hack of behavioral effects of sleep deprivation. TSD and PSD induced genetic damage in rats submitted to pilocarpine-induced SE. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Keywords Epilepsy
Pilocarpine
Sleep deprivation
DNA damage
Locomotor activity
Language English
Sponsor Associacao Fundo de Incentivo a Pesquisa (AFIP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Amparo a Pesquisa do Estado de So Paulo (CEPID)
MCT (INCT-Institut Nacional de Neurociencia Translacional)
ClnAPCe program
Grant number Fundacao de Amparo a Pesquisa do Estado de So Paulo (CEPID): 98/14303-3
Fundacao de Amparo a Pesquisa do Estado de So Paulo (CEPID): 10/15110-8
Fundacao de Amparo a Pesquisa do Estado de So Paulo (CEPID): 09/01030-5
Fundacao de Amparo a Pesquisa do Estado de So Paulo (CEPID): 10/50129-1
Date 2012-05-02
Published in Neuroscience Letters. Clare: Elsevier B.V., v. 515, n. 2, p. 137-140, 2012.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 137-140
Origin http://dx.doi.org/10.1016/j.neulet.2012.03.030
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000303625000008
URI http://repositorio.unifesp.br/handle/11600/34886

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