Glycosaminoglycans from aged human hippocampus have altered capacities to regulate trophic factors activities but not A beta 42 peptide toxicity

Glycosaminoglycans from aged human hippocampus have altered capacities to regulate trophic factors activities but not A beta 42 peptide toxicity

Author Minh Bao Huynh Google Scholar
Villares, Joao Autor UNIFESP Google Scholar
Diaz, Julia Elisa Sepulveda Google Scholar
Christiaans, Stephy Google Scholar
Carpentier, Gilles Google Scholar
Ouidja, Mohand Ouidir Google Scholar
Sissoeff, Ludmilla Google Scholar
Raisman-Vozari, Rita Google Scholar
Papy-Garcia, Dulce Google Scholar
Institution Univ Paris Est
Universidade Federal de São Paulo (UNIFESP)
Univ Paris 06
Abstract Glycosaminoglycans (GAGs) are major extracellular matrix components known to tightly regulate cell behavior by interacting with tissue effectors as trophic factors and other heparin binding proteins. Alterations of GAGs structures might thus modify the nature and extent of these interactions and alter tissue integrity. Here, we studied levels and composition of GAGs isolated from adult and aged human hippocampus and investigated if their changes can influence the function of important trophic factors and the A beta 42 peptide toxicity. Biochemical analyses showed that heparan sulfates are increased in the aged hippocampus. Moreover, GAGs from aged hippocampus showed altered capacities to regulate trophic factor activities without changing their capacities to protect cells from A beta 42 toxicity, compared to adult hippocampus GAGs. Structural alterations in GAGs from elderly were suggested by differential transcripts levels of key biosynthetic enzymes. C5-epimerase and 2-OST expressions were decreased while NDST-2 and 3-OST-4 were increased; in contrast, heparanase expression was unchanged. Results suggest that alteration of GAGs in hippocampus of aged subjects could participate to tissue impairment during aging. (C) 2012 Elsevier Inc. All rights reserved.
Keywords Aging
Hippocampus
Extracellular matrix
Glycosaminoglycans
Heparan sulfate
Growth factors
A beta
Language English
Sponsor French Ministry of Superior Education and Research
Date 2012-05-01
Published in Neurobiology of Aging. New York: Elsevier B.V., v. 33, n. 5, 12 p., 2012.
ISSN 0197-4580 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 12
Origin http://dx.doi.org/10.1016/j.neurobiolaging.2011.09.030
Access rights Closed access
Type Article
Web of Science ID WOS:000302486200025
URI http://repositorio.unifesp.br/handle/11600/34862

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