Lower numbers of natural killer T cells in HIV-1 and Mycobacterium leprae co-infected patients

Lower numbers of natural killer T cells in HIV-1 and Mycobacterium leprae co-infected patients

Author Carvalho, Karina I. Google Scholar
Bruno, Fernanda R. Google Scholar
Snyder-Cappione, Jennifer E. Google Scholar
Maeda, Solange M. Autor UNIFESP Google Scholar
Tomimori, Jane Autor UNIFESP Google Scholar
Xavier, Marilia B. Google Scholar
Haslett, Patrick A. Google Scholar
Nixon, Douglas F. Google Scholar
Kallas, Esper G. Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Univ Calif San Francisco
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Shire Human Genet Therapies
Abstract Natural killer T (NKT) cells are a heterogeneous population of lymphocytes that recognize antigens presented by CD1d and have attracted attention because of their potential role linking innate and adaptive immune responses. Peripheral NKT cells display a memory-activated phenotype and can rapidly secrete large amounts of pro-inflammatory cytokines upon antigenic activation. in this study, we evaluated NKT cells in the context of patients co-infected with HIV-1 and Mycobacterium leprae. the volunteers were enrolled into four groups: 22 healthy controls, 23 HIV-1-infected patients, 20 patients with leprosy and 17 patients with leprosy and HIV-1-infection. Flow cytometry and ELISPOT assays were performed on peripheral blood mononuclear cells. We demonstrated that patients co-infected with HIV-1 and M.leprae have significantly lower NKT cell frequencies [median 0.022%, interquartile range (IQR): 0.0070.051] in the peripheral blood when compared with healthy subjects (median 0.077%, IQR: 0.0320.405, P < 0.01) or HIV-1 mono-infected patients (median 0.072%, IQR: 0.0300.160, P < 0.05). Also, more NKT cells from co-infected patients secreted interferon-? after stimulation with DimerX, when compared with leprosy mono-infected patients (P = 0.05). These results suggest that NKT cells are decreased in frequency in HIV-1 and M.leprae co-infected patients compared with HIV-1 mono-infected patients alone, but are at a more activated state. Innate immunity in human subjects is strongly influenced by their spectrum of chronic infections, and in HIV-1-infected subjects, a concurrent mycobacterial infection probably hyper-activates and lowers circulating NKT cell numbers.
Keywords co-infection
HIV-1
interferon-
leprosy
Mycobacterium leprae
natural killer T cell
Language English
Sponsor National Institutes of Health
Fogarty International Center
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Brazilian Ministry of Science and Technology
New York Community Trust
Grant number National Institutes of Health: R01-AI52731
National Institutes of Health: AI060379
Fogarty International Center: D43 TW00003
FAPESP: 04/15856-9/Kallas
FAPESP: 2010/05845-0/Kallas
Brazilian Ministry of Science and Technology: 484230/2011-5
Date 2012-05-01
Published in Immunology. Malden: Wiley-Blackwell, v. 136, n. 1, p. 96-102, 2012.
ISSN 0019-2805 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 96-102
Origin http://dx.doi.org/10.1111/j.1365-2567.2012.03563.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000302399200011
URI http://repositorio.unifesp.br/handle/11600/34819

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