Immbolization of uricase enzyme in Langmuir and Langmuir-Blodgett films of fatty acids: Possible use as a uric acid sensor

Immbolization of uricase enzyme in Langmuir and Langmuir-Blodgett films of fatty acids: Possible use as a uric acid sensor

Author Zanon, Nathaly Crystyna Muriel Autor UNIFESP Google Scholar
Oliveira, Osvaldo Novais Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Preserving the enzyme structure in solid films is key for producing various bioelectronic devices, including biosensors, which has normally been performed with nanostructured films that allow for control of molecular architectures. in this paper, we investigate the adsorption of uricase onto Langmuir monolayers of stearic acid (SA), and their transfer to solid supports as Langmuir Blodgett (LB) films. Structuring of the enzyme in beta-sheets was preserved in the form of 1-layer LB film, which was corroborated with a higher catalytic activity than for other uricase-containing LB film architectures where the beta-sheets structuring was not preserved. the optimized architecture was also used to detect uric acid within a range covering typical concentrations in the human blood. the approach presented here not only allows for an optimized catalytic activity toward uric acid but also permits one to explain why some film architectures exhibit a superior performance. (C) 2011 Elsevier Inc. All rights reserved.
Keywords Langmuir-Blodgett films
Uricase
Catalytic activity
Air-water interface
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
rede nBioNet (Brazil)
Date 2012-05-01
Published in Journal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 373, p. 69-74, 2012.
ISSN 0021-9797 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 69-74
Origin http://dx.doi.org/10.1016/j.jcis.2011.07.095
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000302208300008
URI http://repositorio.unifesp.br/handle/11600/34812

Show full item record




File

Name: WOS000302208300008.pdf
Size: 747.9Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account