Phylogenetic and host-parasite relationship analysis of Henneguya multiplasmodialis n. sp infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland

Phylogenetic and host-parasite relationship analysis of Henneguya multiplasmodialis n. sp infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland

Author Adriano, Edson Aparecido Autor UNIFESP Google Scholar
Carriero, Mateus Maldonado Google Scholar
Maia, Antonio Augusto Maia Google Scholar
Silva, Marcia Ramos Monteiro da Google Scholar
Naldoni, Juliana Google Scholar
Ceccarelli, Paulo Sergio Google Scholar
Arana, Sarah Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Inst Chico Mendes Conservacao Biodiversidade ICMb
Abstract A new species of the genus Henneguya (Henneguya multiplasmodialis n. sp.) was found infecting the gills of three of 89 specimens (3.3%) of Pseudoplatystoma corruscans and two of 79 specimens (2.6%) of Pseudoplatystoma reticulatum from rivers in the Pantanal wetland, Brazil. Partial sequencing of the 18S rDNA gene of the spores obtained from one plasmodium from the gills of P. corruscans and other one from the gills of P. reticulatum, respectively, resulted in a total of 1560 and 1147 base pairs. As the spores of H. multiplasmodialis n. sp. resemble those of Henneguya corruscans, which is also a parasite of P. corruscans, sequencing of the 18S rDNA gene of the spores of H. corruscans found on P. corruscans caught in the Brazilian Pantanal wetland was also provided to avoid any taxonomic pendency between these two species, resulting in 1913 base pairs. the sequences of H. multiplasmodialis n. sp. parasite of P. corruscans and P. reticulatum and H. corruscans did not match any of the Myxozoa available in the GenBank. the similarity of H. multiplasmodialis n. sp. obtained from P. corruscans to that from P. reticulatum was of 99.7%. Phylogeny revealed a strong tendency among Henneguya species to form clades based on the order and/or family of the host fish. H. multiplasmodialis n. sp. clustered in a clade with Henneguya eirasi and H. corruscans, which are also parasites of siluriforms of the family Pimelodidae and, together with the clade composed of Henneguya spp. parasites of siluriforms of the family Ictaluridae, formed a monophyletic clade of parasites of siluriform hosts. the histological study revealed that the wall of the plasmodia of H. multiplasmodialis n. sp. were covered with a stratified epithelium rich in club cells and supported by a layer of connective tissue. the interior of the plasmodia had a network of septa that divided the plasmodia into numerous compartments. the septa were composed of connective tissue also covered on both sides with a stratified epithelium rich in club cells. Inflammatory infiltrate was found in the tissue surrounding the plasmodia as well as in the septa. (C) 2011 Elsevier B.V. All rights reserved.
Keywords Myxozoa
Club cells
Henneguya corruscans
18S rDNA
Pseudoplatystoma corruscans
Pseudoplatystoma reticulatum
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
CEPTA/ICMBio
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 06/59075-6
Date 2012-04-30
Published in Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 185, n. 2-4, p. 110-120, 2012.
ISSN 0304-4017 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 110-120
Origin http://dx.doi.org/10.1016/j.vetpar.2011.10.008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000302839700008
URI http://repositorio.unifesp.br/handle/11600/34807

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