TOXICITY and RETINAL PENETRATION of INFLIXIMAB in PRIMATES

TOXICITY and RETINAL PENETRATION of INFLIXIMAB in PRIMATES

Author Melo, Gustavo B. Autor UNIFESP Google Scholar
Moraes Filho, Milton N. Autor UNIFESP Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Regatieri, Caio V. Autor UNIFESP Google Scholar
Dreyfuss, Juliana L. Autor UNIFESP Google Scholar
Penha, Fernando M. Autor UNIFESP Google Scholar
Pinheiro, Marcelo M. Autor UNIFESP Google Scholar
Coimbra, Rita C. S. G. Autor UNIFESP Google Scholar
Haapalainen, Edna Freymuller Autor UNIFESP Google Scholar
Farah, Michel E. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: To evaluate the retinal penetration and toxicity of two doses of intravitreal infliximab in primates.Methods: Ten marmosets (Callithrix jacchus) were given intravitreal injection of 100 mu g or 400 mu g of infliximab, and balanced salt solution served as control. At baseline and after 24 hours (5 animals) and 7 days (the other 5), the eyes were examined by electroretinography. They were then killed (at 24 hours and 7 days) and assessed by light microscopy and transmission electron microscopy for toxicity and immunohistochemistry, using a biotinylated anti-human immunoglobulin G, to evaluate retinal penetration.Results: There was no difference over 50% of the electroretinography b-wave between baseline and the time points studied in all animals. Light and electron microscopy, and electroretinography analysis, showed no signs of toxicity in any of the animals. Strong presence of infliximab was observed in all retinal layers 7 days after intravitreal injection at both doses (100 and 400 mu g).Conclusion: Infliximab at doses of 100 and 400 mu g seemed to cause no damage to the retina 24 hours and 7 days after its intravitreal injection, and deeply penetrated all its layers, in primates. These results encourage future perspectives for the treatment of chronic inflammatory diseases of the retina in humans. RETINA 32: 606-612, 2012
Keywords electron microscopy
electroretinography
infliximab
retinal penetration
toxicity
Language English
Sponsor Pan-American Ophthalmological Foundation
Date 2012-03-01
Published in Retina-the Journal of Retinal and Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 32, n. 3, p. 606-612, 2012.
ISSN 0275-004X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 606-612
Origin http://dx.doi.org/10.1097/IAE.0b013e3182252a23
Access rights Closed access
Type Article
Web of Science ID WOS:000300907200025
URI http://repositorio.unifesp.br/handle/11600/34690

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