Cytogenetic instability of dental pulp stem cell lines

Cytogenetic instability of dental pulp stem cell lines

Author Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Kulikowski, Leslie Domenici Google Scholar
Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Nunes Lipay, Monica Vannucci Autor UNIFESP Google Scholar
Melaragno, Maria Isabel Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Vacanti, Joseph Phillip Google Scholar
Yelick, Pamela Crotty Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
INCT Biofabris
Massachusetts Gen Hosp
Harvard Univ
Dept Surg
Tufts Univ
Abstract Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.
Keywords Chromosomal abnormalities
Cell transplantation
Tooth tissue engineering
Human dental stem cells
Language English
Sponsor INCT-Biofabrication Institute
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED
Grant number CNPq: 573661/2008-1
FAPESP: 08/57860-3
FAPESP: 07-58856-7
FAPESP: 07-51227-4
FAPESP: 07-59488-1
Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED: 208RT0340
NIH/NIDCR: R01DE016132
NIH/NIDCR: R03TW007665
Date 2012-02-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 43, n. 1, p. 89-94, 2012.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 89-94
Access rights Closed access
Type Article
Web of Science ID WOS:000299376400011

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