Author |
Duailibi, Monica Talarico
![]() ![]() Kulikowski, Leslie Domenici ![]() Duailibi, Silvio Eduardo ![]() ![]() Nunes Lipay, Monica Vannucci ![]() ![]() Melaragno, Maria Isabel ![]() Ferreira, Lydia Masako ![]() ![]() Vacanti, Joseph Phillip ![]() Yelick, Pamela Crotty ![]() |
Institution | Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) INCT Biofabris Massachusetts Gen Hosp Harvard Univ Dept Surg Tufts Univ |
Abstract | Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies. |
Keywords |
Chromosomal abnormalities
Cell transplantation Tooth tissue engineering Human dental stem cells |
Language | English |
Sponsor | INCT-Biofabrication Institute Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Rede Biofab, Ibero-American Network of Biofabrication-BIOFAB-CYTED NIH/NIDCR |
Grant number |
|
Date | 2012-02-01 |
Published in | Journal of Molecular Histology. Dordrecht: Springer, v. 43, n. 1, p. 89-94, 2012. |
ISSN | 1567-2379 (Sherpa/Romeo, impact factor) |
Publisher | Springer |
Extent | 89-94 |
Origin |
|
Access rights | Closed access |
Type | Article |
Web of Science ID | WOS:000299376400011 |
URI | http://repositorio.unifesp.br/handle/11600/34566 |
File | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |