First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid

Author Rabello, Michelle Christiane da Silva Autor UNIFESP Google Scholar
Matsumoto, Cristianne Kayoko Autor UNIFESP Google Scholar
Almeida, Luiz Gonzaga Paula de Google Scholar
Carmen Menendez, Maria Google Scholar
Oliveira, Rosangela Siqueira de Google Scholar
Silva, Rosa Maria Autor UNIFESP Google Scholar
Jesus Garcia, Maria Google Scholar
Leão, Sylvia Cardoso Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Lab Nacl Comp Cient
Univ Autonoma Madrid
Inst Adolfo Lutz Registro
Abstract Background: in a previous study, we detected the presence of a Mycobacterium avium species-specific insertion sequence, IS1245, in Mycobacterium kansasii. Both species were isolated from a mixed M. avium-M. kansasii bone marrow culture from an HIV-positive patient. the transfer mechanism of this insertion sequence to M. kansasii was investigated here.Methodology/Principal Findings: A linear plasmid (pMA100) was identified in all colonies isolated from the M. avium-M. kansasii mixed culture carrying the IS1245 element. the linearity of pMA100 was confirmed. Other analyses suggested that pMA100 contained a covalently bound protein in the terminal regions, a characteristic of invertron linear replicons. Partial sequencing of pMA100 showed that it bears one intact copy of IS1245 inserted in a region rich in transposase-related sequences. These types of sequences have been described in other linear mycobacterial plasmids. Mating experiments were performed to confirm that pMA100 could be transferred in vitro from M. avium to M. kansasii. pMA100 was transferred by in vitro conjugation not only to the M. kansasii strain from the mixed culture, but also to two other unrelated M. kansasii clinical isolates, as well as to Mycobacterium bovis BCG Moreau.Conclusions/Significance: Horizontal gene transfer (HGT) is one of most important mechanisms leading to the evolution and diversity of bacteria. This work provides evidence for the first time on the natural occurrence of HGT between different species of mycobacteria. Gene transfer, mediated by a novel conjugative plasmid, was detected and experimentally reproduced.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Cooperacion Interuniversitaria UAM-Banco Santander con America Latina (CEAL), UAM, Spain
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: FAPESP - 06/01533-9
Date 2012-01-03
Published in Plos One. San Francisco: Public Library Science, v. 7, n. 1, 8 p., 2012.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 8
Origin http://dx.doi.org/10.1371/journal.pone.0029884
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000301123400109
URI http://repositorio.unifesp.br/handle/11600/34522

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