Suppression of Inflammatory Cytokine Secretion by an NF-kappa B Inhibitor DHMEQ in Nasal Polyps Fibroblasts

Suppression of Inflammatory Cytokine Secretion by an NF-kappa B Inhibitor DHMEQ in Nasal Polyps Fibroblasts

Author Valera, Fabiana Cardoso P. Autor UNIFESP Google Scholar
Umezawa, Kazuo Google Scholar
Brassesco, Maria S. Google Scholar
Castro-Gamero, Angel M. Google Scholar
Queiroz, Rosane G. R. Google Scholar
Scrideli, Carlos A. Google Scholar
Tone, Luiz G. Google Scholar
Anselmo-Lima, Wilma T. Google Scholar
Institution Universidade de São Paulo (USP)
Keio Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: NF-kappa B is an essential transcription factor strongly associated to inflammatory response in chronic rhinosinusitis with nasal polyps (CRSwNP). DHMEQ is a NF-kappa B inhibitor that has been previously described with a greatpotential indecreasing inflammation in diseases other than CRSwNP. the aim of study isto evaluate the ability of DHMEQ to reducethe inflammatory recruiters on CRSwNP and to compare its anti-inflammatory profile as a single-agent or in association with fluticasone propionate (FP). Methods: nasal polyp fibroblasts were cultured in TNF-alpha enriched media. Cells were submitted to three different concentrations (1, 10 and 100nM) of either FP, DHMEQ or both. Inflammatory response was accessed by VCAM-1, ICAM-1 and RANTES expression (by RTQ-PCR) and protein levels by ELISA. Nuclear translocation of NF-kappa B was also evaluated. Results: both FP and DHMEQ inhibited inflammatory recruiters' production and NF-kappa B nuclear translocation. Interestingly, the anti-inflammatory effect from the association steroids plus DHMEQ was more intense than of each drug in separate. Conclusion: DHMEQ seems efficient in modulating the inflammatory process in CRSwNP. the synergic anti-inflammatory effect of DHMEQ and steroids may be a promising strategy to be explored, particularly in the setting of steroid-resistant NP. Copyright (c) 2012 S. Karger AG, Basel
Keywords DHMEQ
Fluticasone propionate
Glucocorticoid
Nasal polyps
NF-kappaB
Rhinosinusitis
Dehydroxymethylepoxyquinomicin
In vitro culture
Polyps fibroblasts
Topical steroid
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
IRB
Grant number FAPESP: 07/50359-4
IRB: 4374/2007
Date 2012-01-01
Published in Cellular Physiology and Biochemistry. Basel: Karger, v. 30, n. 1, p. 13-22, 2012.
ISSN 1015-8987 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 13-22
Origin http://dx.doi.org/10.1159/000339042
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000305585400002
URI http://repositorio.unifesp.br/handle/11600/34387

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