Impact of birth in the presence and absence of simulated birth injury on vaginal glycosaminoglycan content

Impact of birth in the presence and absence of simulated birth injury on vaginal glycosaminoglycan content

Author Ruano, Jose Maria Autor UNIFESP Google Scholar
Feldner, Paulo Cezar Autor UNIFESP Google Scholar
Takano, Claudia Cristina Autor UNIFESP Google Scholar
Castro, Rodrigo Aquino Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Ferreira Sartori, Marair Gracio Autor UNIFESP Google Scholar
Batista Castello Girao, Manoel Joao Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract This study aims to evaluate the effects of simulated birth trauma and vaginal and Cesarean delivery on glycosaminoglycans (GAGs) in the vagina of female rats.One hundred ten rats were divided into six groups: A (control), B (vaginal trauma), C (Cesarean delivery), D (Cesarean delivery followed by vaginal trauma), E (vaginal delivery), and F (20th day of gestation). in each group, half of the animals were killed 4 days after the procedure (time 1) and 12 weeks later (time 2). GAGs were extracted, isolated, and identified by using agarose gel electrophoresis and quantified by densitometry. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney tests.We observed a significant decrease in total GAGs and dermatan sulfate (DS) at time 1. Evaluation at time 2 showed a significant increase in total GAGs, DS, and heparan sulfate.Levels of sulfated GAGs in the rat vagina are affected by delivery and simulated birth trauma.
Keywords Birth trauma
Glycosaminoglycans
Pregnancy
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-12-01
Published in International Urogynecology Journal. London: Springer London Ltd, v. 22, n. 12, p. 1513-1519, 2011.
ISSN 0937-3462 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1513-1519
Origin http://dx.doi.org/10.1007/s00192-011-1514-0
Access rights Closed access
Type Article
Web of Science ID WOS:000296647000008
URI http://repositorio.unifesp.br/handle/11600/34272

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