alpha-Tocopherol induces hematopoietic stem/progenitor cell expansion and ERK1/2-mediated differentiation

alpha-Tocopherol induces hematopoietic stem/progenitor cell expansion and ERK1/2-mediated differentiation

Author Nogueira-Pedro, Amanda Autor UNIFESP Google Scholar
Barbosa, Christiano M. V. Autor UNIFESP Google Scholar
Segreto, Helena Regina Comodo Autor UNIFESP Google Scholar
Lungato, Lisandro Autor UNIFESP Google Scholar
D'Almeida, Vania Autor UNIFESP Google Scholar
Moraes, Andrea Aparecida F. S. Autor UNIFESP Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Tocopherols promote or inhibit growth in different cell types. in the hematopoietic system, the radioprotective property of tocopherols is thought to act through the expansion of primitive hematopoietic cells. However, the mechanisms activated by tocopherols and which HPs are affected remain poorly understood. To better address these questions, mice were treated with alpha-tocopherol, and its effects were investigated in the BM microenvironment. alpha-Tocopherol induced increased proliferation in HSC/HP cells, leading to BM hyperplasia. in addition, differentiation to the granulocytic/monocytic lineage was enhanced by alpha-tocopherol treatment. alpha-Tocopherol treatment resulted in decreased basal phosphorylation of ERK1/2, PKC, and STAT-5 in HSC/HP cells. in contrast, alpha-tocopherol enhanced ERK1/2 activation in response to IL-3 stimulation in HSC/HP cells without altering the expression of IL-3Rs. Moreover, alpha-tocopherol-induced differentiation and ERK1/2 activation were abolished in mice pretreated with a MEK inhibitor (PD98059); however, pretreatment with PD98059 did not reduce the alpha-tocopherol-mediated increase in HSC/HP cells but instead, further enhanced their proliferation. Therefore, alpha-tocopherol induces expansion of HSC/HP cells by a nonidentified intracellular pathway and granulocytic/monocytic differentiation through ERK1/2 activation. J. Leukoc. Biol. 90: 1111-1117; 2011.
Keywords proliferation
IL-3
monocyte
granulocyte
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-12-01
Published in Journal of Leukocyte Biology. Bethesda: Federation Amer Soc Exp Biol, v. 90, n. 6, p. 1111-1117, 2011.
ISSN 0741-5400 (Sherpa/Romeo, impact factor)
Publisher Federation Amer Soc Exp Biol
Extent 1111-1117
Origin http://dx.doi.org/10.1189/jlb.0611282
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000298154400009
URI http://repositorio.unifesp.br/handle/11600/34261

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