Size and specificity of radiopharmaceuticals for sentinel lymph node detection

Size and specificity of radiopharmaceuticals for sentinel lymph node detection

Author Fernandez Nunez, Eutimio Gustavo Google Scholar
Teodoro, Rodrigo Google Scholar
Wiecek, Danielle Pereira Google Scholar
Silva, Natanael Gomes da Google Scholar
Martinelli, Jose Roberto Google Scholar
Oliveira Filho, Renato Santos de Autor UNIFESP Google Scholar
Institution Inst Energet & Nucl Res
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Biological performance of radiotracers for sentinel node detection analyzed in the light of molecular design and dimension is not widely available.Purpose: To evaluate the effect of dextran molecular size and the presence of tissue-binding units (mannose) within the model of (99m)Tc-carbonyl conjugate for sentinel lymph node detection.Material and Methods: Four dextran conjugates with and without mannose in the chemical backbone were included. All polymers were radiolabeled using the precursor [(99m)Tc(OH(2))(3)(CO)(3)](+). Radio labeling conditions targeted the best radiochemical purity and specific activity for each radiopharmaceutical, and partition coefficients were also defined. Lymphoscintigraphy and ex-vivo biodistribution in popliteal lymph node, liver and kidneys were performed in Wistar rats. the effects of molecular weight and mannose presence were assessed by a two-level factorial design.Results: Radiochemical purity was indirectly related to molecular weight and presence of mannose in the polymer structure. All products were able to detect popliteal lymph node, however, uptake was strongly influenced by use of mannose (4-fold higher). Excretion was similarly modulated by differences in molecular weight. Mannose-enhanced lymph node uptake and higher molecule size in the range under study benefitted lymphoscintigraphic performance.Conclusion: Screening of radiopharmaceuticals for lymphoscintigraphy might improve with attention to the mentioned physico-chemical features of the molecule.
Keywords Radiopharmaceutical
sentinel lymph node
technetium
pharmacology
mannose receptors
Language English
Sponsor National Commission for Nuclear Energy (CNEN, São Paulo, Brazil)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
International Atomic Energy Agency (IAEA, Vienna, Austria)
Date 2011-09-01
Published in Acta Radiologica. London: Royal Soc Medicine Press Ltd, v. 52, n. 7, p. 774-778, 2011.
ISSN 0284-1851 (Sherpa/Romeo, impact factor)
Publisher Royal Soc Medicine Press Ltd
Extent 774-778
Origin http://dx.doi.org/10.1258/ar.2011.100506
Access rights Closed access
Type Article
Web of Science ID WOS:000295759600016
URI http://repositorio.unifesp.br/handle/11600/34037

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