Application of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children

Application of Real-Time PCR Stool Assay for Helicobacter pylori Detection and Clarithromycin Susceptibility Testing in Brazilian Children

Author Scaletsky, Isabel C. A. Autor UNIFESP Google Scholar
Aranda, Katia R. S. Autor UNIFESP Google Scholar
Garcia, Gabriela T. Autor UNIFESP Google Scholar
Goncalves, Manoel E. P. Google Scholar
Cardoso, Silvia R. Google Scholar
Iriya, Kiyoshi Google Scholar
Silva, Neusa P. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background: Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens.Objective: the aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children.Methods: DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing.Results: the sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance.Conclusion: This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
Keywords Helicobacter pylori
stool PCR
children
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-08-01
Published in Helicobacter. Malden: Wiley-Blackwell, v. 16, n. 4, p. 311-315, 2011.
ISSN 1083-4389 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 311-315
Origin http://dx.doi.org/10.1111/j.1523-5378.2011.00845.x
Access rights Closed access
Type Article
Web of Science ID WOS:000292867900008
URI http://repositorio.unifesp.br/handle/11600/33943

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