Endoplasmic Reticulum Calcium Release Engages Bax Translocation in Cortical Astrocytes

Endoplasmic Reticulum Calcium Release Engages Bax Translocation in Cortical Astrocytes

Author Morales, A. P. Google Scholar
Carvalho, A. C. P. Google Scholar
Monteforte, P. T. Google Scholar
Hirata, H. Google Scholar
Han, S. W. Autor UNIFESP Google Scholar
Hsu, Y. -T. Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Med Univ S Carolina
Abstract Apoptosis is a highly complex form of cell death that can be triggered by alterations in Ca(2+) homeostasis. Members of the Bcl-2 family may regulate apoptosis and modulate Ca(2+) distribution within intracellular compartments. Bax, a proapoptotic member of the family, is constitutively expressed and soluble in the cytosol and, under apoptotic induction, translocates to mitochondrial membranes. However, it is not clear if the intracellular Ca(2+) stores and selective Ca(2+) releases can modulate or control Bax translocation. the aim of this study was to investigate the relation of intracellular Ca(2+) stores with Bax translocation in rat cortical astrocytes. Results show that the classical apoptotic inducer, staurosporine, caused high elevations of cytosolic Ca(2+) that precede Bax translocation. On the other hand, agents that mobilize Ca(2+) from endoplasmic reticulum such as noradrenaline or thapsigargin, induced Bax translocation, while mitochondrial Ca(2+) release evoked by carbonyl cyanide-p-(trifluoromethoxyphenyl) hydrazone was not able to cause Bax punctation. in addition, microinjection of inositol 1,4,5- trisphosphate induced Bax translocation. Taken together, our results show that in Bax overexpressing cortical astrocytes, endoplasmic reticulum-Ca(2+) release may induce Bax transactivation and specifically control apoptosis.
Keywords Bax
Calcium
Endoplasmic reticulum
Mitochondria
Apoptosis
Astrocytes
Rat
Cell death
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
NIH
Grant number NIH: NS40932
Date 2011-05-01
Published in Neurochemical Research. New York: Springer/plenum Publishers, v. 36, n. 5, p. 829-838, 2011.
ISSN 0364-3190 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 829-838
Origin http://dx.doi.org/10.1007/s11064-011-0411-8
Access rights Closed access
Type Article
Web of Science ID WOS:000289215000017
URI http://repositorio.unifesp.br/handle/11600/33670

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