Investigation of the Europium Emission Spectra of the Europium-Oxytetracycline Complex in the Presence of Human Low-Density Lipoproteins

Investigation of the Europium Emission Spectra of the Europium-Oxytetracycline Complex in the Presence of Human Low-Density Lipoproteins

Author Santos Teixeira, Luciane dos Google Scholar
Grasso, Andrea Nastri Google Scholar
Monteiro, Andrea Moreira Google Scholar
Figueiredo Neto, Antonio M. Google Scholar
Vieira, Nilson Dias Google Scholar
Gidlund, Magnus Google Scholar
Steffens, Juliana Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
IPEN CNEN SP
Universidade de São Paulo (USP)
Abstract Low-Density Lipoprotein (LDL), often known as bad cholesterol is one of the responsible to increase the risk of coronary arterial diseases. for this reason, the cholesterol present in the LDL particle has become one of the main parameters to be quantified in routine clinical diagnosis. A number of tools are available to assess LDL particles and estimate the cholesterol concentration in the blood. the most common methods to quantify the LDL in the plasma are the density gradient ultracentrifugation and nuclear magnetic resonance (NMR). However, these techniques require special equipments and can take a long time to provide the results. in this paper, we report on the increase of the Europium emission in Europium-oxytetracycline complex aqueous solutions in the presence of LDL. This increase is proportional to the LDL concentration in the solution. This phenomenum can be used to develop a method to quantify the number of LDL particles in a sample. A comparison between the performances of the oxytetracycline and the tetracycline in the complexes is also made.
Keywords LDL
Europium
Fluorescence
Biosensor
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
INCT-FCx (Instituto Nacional de Ciencias e Tecnologia de Fluidos Complexos)
Date 2011-05-01
Published in Journal of Fluorescence. New York: Springer/plenum Publishers, v. 21, n. 3, p. 887-892, 2011.
ISSN 1053-0509 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 887-892
Origin http://dx.doi.org/10.1007/s10895-010-0602-8
Access rights Closed access
Type Article
Web of Science ID WOS:000291999400008
URI http://repositorio.unifesp.br/handle/11600/33668

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